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Applied and Environmental Microbiology, November 1999, p. 4725-4728, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antiproliferative Effects of Homogenates Derived from Five Strains of Candidate Probiotic Bacteria

Tanja Pessi,1,* Yelda Sütas,1 Maija Saxelin,2 Harri Kallioinen,2 and Erika Isolauri1

Department of Pediatrics, University of Turku, Turku,1 and Valio Ltd., Research and Development Centre, Helsinki,2 Finland

Received 14 April 1999/Accepted 7 August 1999

Unheated and heat-treated homogenates were separately prepared from candidate probiotic bacteria, including Lactobacillus rhamnosus GG, Bifidobacterium lactis, Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Streptococcus thermophilus. We compared the phytohemagglutinin-induced proliferation of mononuclear cells in the presence of homogenates and in the presence of a control containing no homogenate by assessing thymidine incorporation in cell cultures. All homogenates suppressed proliferation, whether the enzymatic activity was inactivated or not inactivated by heating. When the proliferation assays were repeated with cytoplasmic and cell wall extracts derived from the homogenate of L. rhamnosus GG, the cytoplasmic extract but not the cell wall extract was suppressive. These findings indicate that candidate probiotic bacteria possess a heat-stable antiproliferative component(s). These bacteria may be used to generate microbiologically nonviable yet immunologically active probiotic food products that are easier to store and have a longer shelf life.

* Corresponding author. Mailing address: Department of Pediatrics, University of Turku, 20520 Turku, Finland. Phone: 358-10-381 3217. Fax: 358-10-381 3219. E-mail: tanja.pessi{at}valio.fi.

Applied and Environmental Microbiology, November 1999, p. 4725-4728, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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