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Applied and Environmental Microbiology, November 1999, p. 4793-4798, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transposon-Induced Mutations in Two Loci of Listeria monocytogenes Serotype 1/2a Result in Phage Resistance and Lack of N-Acetylglucosamine in the Teichoic Acid of the Cell Wall

Huyen L. Tran,1 F. Fiedler,2 D. A. Hodgson,3 and S. Kathariou1,*

Department of Microbiology, University of Hawaii, Honolulu, Hawaii 968221; Institute of Genetics and Microbiology, University of Munich, Munich, Germany2; and Department of Biological Sciences, University of Warwick, Coventry, West Midlands, United Kingdom3

Received 12 April 1999/Accepted 9 July 1999

Teichoic acid-associated N-acetylglucosamine and rhamnose have been shown to serve as phage receptors in Listeria monocytogenes serotype 1/2a. We generated and characterized two single-copy Tn916Delta E mutants which were resistant to phage A118 and several other serotype 1/2a-specific phages. In one mutant the insertion was immediately upstream of the recently identified ptsHI locus, which encodes two proteins of the phosphoenolpyruvate-dependent carbohydrate uptake system, whereas in the other the insertion was immediately upstream of an operon whose most distal gene was clpC, involved in stress responses and virulence. Transduction experiments confirmed the association of the phage-resistant phenotype of these mutants with the transposon insertion. Phage A118 resistance of the mutants could be attributed to inability of the phage to adsorb onto the mutant cells, and biochemical analysis of cell wall composition showed that the teichoic acids of both mutants were deficient in N-acetylglucosamine. Rhamnose and other teichoic acid and cell wall components were not affected.

* Corresponding author. Mailing address: Department of Microbiology, University of Hawaii, 2538 The Mall, Snyder Hall Rm. 207, Honolulu, HI 96822. Phone: (808) 956-8015. Fax: (808) 956-5339. E-mail: ksophia{at}hawaii.edu.

Applied and Environmental Microbiology, November 1999, p. 4793-4798, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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