This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parker, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parker, M. A.
Agricola
Right arrow Articles by Parker, M. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 1999, p. 4914-4920, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Relationships of Bradyrhizobia from the Legumes Apios americana and Desmodium glutinosum

Matthew A. Parker*

Department of Biological Sciences, State University of New York, Binghamton, New York 13902

Received 10 May 1999/Accepted 24 August 1999

Multilocus enzyme electrophoresis, partial 23S rRNA sequences, and nearly full-length 16S rRNA sequences all indicated high genetic similarity among root-nodule bacteria associated with Apios americana, Desmodium glutinosum, and Amphicarpaea bracteata, three common herbaceous legumes whose native geographic ranges in eastern North America overlap extensively. A total of 19 distinct multilocus genotypes (electrophoretic types [ETs]) were found among the 35 A. americana and 33 D. glutinosum isolates analyzed. Twelve of these ETs (representing 78% of all isolates) were either identical to ETs previously observed in A. bracteata populations, or differed at only one locus. Within both 23S and 16S rRNA genes, several isolates from A. americana and D. glutinosum were either identical to A. bracteata isolates or showed only single nucleotide differences. Growth rates and nitrogenase activities of A. bracteata plants inoculated with isolates from D. glutinosum were equivalent to levels found with native A. bracteata bacterial isolates, but none of the three A. americana isolates tested had high symbiotic effectiveness on A. bracteata. Phylogenetic analysis of both 23S and 16S rRNA sequences indicated that both A. americana and D. glutinosum harbored rare bacterial genotypes similar to Bradyrhizobium japonicum USDA 110. However, the predominant root nodule bacteria on both legumes were closely related to Bradyrhizobium elkanii.

* Mailing address: Department of Biological Sciences, State University of New York, Binghamton, NY 13902. Phone: (607) 777-6283. Fax: (607) 777-6521. E-mail: mparker{at}binghamton.edu.

Applied and Environmental Microbiology, November 1999, p. 4914-4920, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Andam, C. P., Parker, M. A. (2007). Novel Alphaproteobacterial Root Nodule Symbiont Associated with Lupinus texensis. Appl. Environ. Microbiol. 73: 5687-5691 [Abstract] [Full Text]  
  • Itoh, K., Tashiro, Y., Uobe, K., Kamagata, Y., Suyama, K., Yamamoto, H. (2004). Root Nodule Bradyrhizobium spp. Harbor tfdA{alpha} and cadA, Homologous with Genes Encoding 2,4-Dichlorophenoxyacetic Acid-Degrading Proteins. Appl. Environ. Microbiol. 70: 2110-2118 [Abstract] [Full Text]  
  • Parker, M. A., Lafay, B., Burdon, J. J., van Berkum, P. (2002). Conflicting phylogeographic patterns in rRNA and nifD indicate regionally restricted gene transfer in Bradyrhizobium. Microbiology 148: 2557-2565 [Abstract] [Full Text]  
  • Parker, M. A. (2002). Bradyrhizobia from Wild Phaseolus, Desmodium, and Macroptilium Species in Northern Mexico. Appl. Environ. Microbiol. 68: 2044-2048 [Abstract] [Full Text]  
  • Simms, E. L., Taylor, D. L. (2002). Partner Choice in Nitrogen-Fixation Mutualisms of Legumes and Rhizobia. Integr. Comp. Biol. 42: 369-380 [Abstract] [Full Text]  
  • Parker, M. A. (2001). Case of Localized Recombination in 23S rRNA Genes from Divergent Bradyrhizobium Lineages Associated with Neotropical Legumes. Appl. Environ. Microbiol. 67: 2076-2082 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»