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Applied and Environmental Microbiology, November 1999, p. 4981-4986, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Lactobacillus salivarius CTC2197 Prevents Salmonella enteritidis Colonization in Chickens

Mònica Pascual,1 Marta Hugas,1 Jose Ignacio Badiola,2 Josep Maria Monfort,1 and Margarita Garriga1,*

Institute for Food and Agricultural Research and Technology (IRTA), Meat Technology Center-CeRTA, 17121 Monells,1 and Animal Health Unit, 08004 Barcelona,2 Spain

Received 22 April 1999/Accepted 26 August 1999

A rifampin-resistant Lactobacillus salivarius strain, CTC2197, was assessed as a probiotic in poultry, by studying its ability to prevent Salmonella enteritidis C-114 colonization in chickens. When the probiotic strain was dosed by oral gavage together with S. enteritidis C-114 directly into the proventriculus in 1-day-old Leghorn chickens, the pathogen was completely removed from the birds after 21 days. The same results were obtained when the probiotic strain was also administered through the feed and the drinking water apart from direct inoculation into the proventriculus. The inclusion of L. salivarius CTC2197 in the first day chicken feed revealed that a concentration of 105 CFU g-1 was enough to ensure the colonization of the gastrointestinal tract of the birds after 1 week. However, between 21 and 28 days, L. salivarius CTC2197 was undetectable in the gastrointestinal tract of some birds, showing that more than one dose would be necessary to ensure its presence till the end of the rearing time. Freeze-drying and freezing with glycerol or skim milk as cryoprotective agents, appeared to be suitable methods to preserve the probiotic strain. The inclusion of the L. salivarius CTC2197 in a commercial feed mixture seemed to be a good way to supply it on the farm, although the strain showed sensitivity to the temperatures used during the feed mixture storage and in the chicken incubator rooms. Moreover, survival had been improved after several reinoculations in chicken feed mixture.

* Corresponding author. Mailing address: Institute for Food and Agricultural Research and Technology (IRTA), Meat Technology Center-CeRTA, Granja Camps i Armet, 17121 Monells, Spain. Phone: 34-972 630052. Fax: 34-972 630373. E-mail: margarita.garriga{at}irta.es.

Applied and Environmental Microbiology, November 1999, p. 4981-4986, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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