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Applied and Environmental Microbiology, November 1999, p. 5142-5147, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Display of Polyhistidine Peptides on the Escherichia coli Cell Surface by Using Outer Membrane Protein C as an Anchoring Motif

Zhaohui Xu1,2 and Sang Yup Lee1,*

Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon 305-701, Korea,1 and Department of Microbial Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China2

Received 11 May 1999/Accepted 16 August 1999

A novel cell surface display system was developed by employing Escherichia coli outer membrane protein C (OmpC) as an anchoring motif. Polyhistidine peptides consisting of up to 162 amino acids could be successfully displayed on the seventh exposed loop of OmpC. Recombinant cells displaying polyhistidine could adsorb up to 32.0 µmol of Cd2+ per g (dry weight) of cells.

* Corresponding author. Mailing address: Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea. Phone: 042-869-3930. Fax: 042-869-8800. E-mail: leesy{at}sorak.kaist.ac.kr.

Applied and Environmental Microbiology, November 1999, p. 5142-5147, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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