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Applied and Environmental Microbiology, November 1999, p. 5151-5153, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR Amplification of the Gene acmA Differentiates Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris

Sonia Garde, Mar Babin, Pilar Gaya, Manuel Nuñez, and Margarita Medina*

Departamento de Tecnología de Alimentos, INIA, 28040 Madrid, Spain

Received 16 February 1999/Accepted 16 July 1999

The occurrence of the acmA gene, encoding the lactococcal N-acetylmuramidase in new lactococcal isolates from raw milk cheeses, has been determined. Isolates were genotypically identified to the subspecies level with a PCR technique. On the basis of PCR amplification of the acmA gene, the presence or absence of an additional amplicon of approximately 700 bp correlated with Lactococcus lactis subspecies. L. lactis subsp. lactis exhibits both the expected 1,131-bp product and the additional amplicon, whereas L. lactis subsp. cremoris exhibits a single 1,131-bp fragment.

* Corresponding author. Mailing address: Departamento de Tecnología de Alimentos, INIA, Carretera de La Coruña km 7, 28040 Madrid, Spain. Phone: 34 91 3476774. Fax: 34 91 3572293. E-mail: mmedina{at}inia.es.

Applied and Environmental Microbiology, November 1999, p. 5151-5153, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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