AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cappelier, J. M.
Right arrow Articles by Federighi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cappelier, J. M.
Right arrow Articles by Federighi, M.
Agricola
Right arrow Articles by Cappelier, J. M.
Right arrow Articles by Federighi, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 1999, p. 5154-5157, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Recovery in Embryonated Eggs of Viable but Nonculturable Campylobacter jejuni Cells and Maintenance of Ability To Adhere to HeLa Cells after Resuscitation

J. M. Cappelier,1,* J. Minet,2 C. Magras,1 R. R. Colwell,3 and M. Federighi1

Unité Associé INRA d'Hygiène Alimentaire, Ecole Nationale Vétérinaire de Nantes, 44307 Nantes,1 and Faculté de Pharmacie, Laboratoire de Microbiologie, 35043 Rennes,2 France, and Biotechnology Institute, University of Maryland, College Park, Maryland 207403

Received 19 October 1998/Accepted 16 August 1999

The existence of a viable but nonculturable (VBNC) state has been described for Campylobacter jejuni as it had been for a number pathogenic bacteria. Three C. jejuni human isolates were suspended in surface water and subsequently entered the VBNC state. After starvation for 30 days, VBNC cells were inoculated in the yolk sacs of embryonated eggs. Culturable cells were detected in a large proportion of the embryonated eggs inoculated with VBNC C. jejuni cells. Recovered cells kept their adhesion properties.

* Corresponding author. Mailing address: Unité Associé INRA d'Hygiène Alimentaire, Ecole Nationale Vétérinaire de Nantes, B.P. 40706, 44307 Nantes, France. Phone: (33)-02-40-68-76-82. Fax: (33)-02-40-68-77-62. E-mail: cappelier{at}vet-nantes.fr.

Applied and Environmental Microbiology, November 1999, p. 5154-5157, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.