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Applied and Environmental Microbiology, November 1999, p. 5182-5185, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genotypic Diversity among Brevibacillus laterosporus Strains

Viviane Zahner,1,* Leon Rabinovitch,2 Phylip Suffys,1 and Hooman Momen1

Laboratory of Systematic Biochemistry, Department of Biochemistry and Molecular Biology,1 and Laboratory of Bacterial Physiology, Department of Bacteriology,2 Oswaldo Cruz Institute-FIOCRUZ, Rio de Janeiro, RJ, Brazil

Received 13 April 1999/Accepted 27 August 1999

In comparison with other entomopathogenic Bacillus species, the genome of Brevibacillus laterosporus is poorly characterized. The aim of this study was to examine genetic variability in B. laterosporus by using a range of typing methodologies. Strains of B. laterosporus were examined for variation in 13 chromosomal genes encoding enzymes by multilocus enzyme electrophoresis. Optimal conditions of pulsed-field gel electrophoresis and randomly amplified polymorphic DNA were established that allowed analysis of the genome of B. laterosporus. None of these techniques allowed the identification of a convenient molecular marker for entomopathogenic strains, although one specific primer amplified only DNA from almost all mosquitocidal strains.

* Corresponding author. Mailing address: Laboratory of Systematic Biochemistry, Department of Biochemistry and Molecular Biology, Oswaldo Cruz Institute-FIOCRUZ, Manguinhos CEP 21045-900 Rio de Janeiro, RJ, Brazil. Phone: 55-21-2907549. Fax: 55-21-5903495. E-mail: vzahner{at}gene.dbbm.fiocruz.br.

Applied and Environmental Microbiology, November 1999, p. 5182-5185, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • de Oliveira, E. J., Rabinovitch, L., Monnerat, R. G., Passos, L. K. J., Zahner, V. (2004). Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control. Appl. Environ. Microbiol. 70: 6657-6664 [Abstract] [Full Text]  


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