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Applied and Environmental Microbiology, December 1999, p. 5252-5256, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Disruption of TRI101, the Gene Encoding Trichothecene 3-O-Acetyltransferase, from Fusarium sporotrichioides

Susan P. McCormick,1,* Nancy J. Alexander,1 Susan E. Trapp,2 and Thomas M. Hohn1,dagger

Mycotoxin Research Unit, USDA/ARS National Center for Agricultural Utilization Research, Peoria, Illinois 61604,1 and Department of Chemistry, University of Maryland, College Park, Maryland 207422

Received 10 March 1999/Accepted 10 September 1999

We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3-O-acetyltransferase that is the F. sporotrichioides homolog of the Fusarium graminearum TRI101 gene. Mutants of F. sporotrichioides NRRL 3299 produced by disruption of TRI101 were altered in their abilities to synthesize T-2 toxin and accumulated isotrichodermol and small amounts of 3,15-didecalonectrin and 3-decalonectrin, trichothecenes that are not observed in cultures of the parent strain. Our results indicate that TRI101 converts isotrichodermol to isotrichodermin and is required for the biosynthesis of T-2 toxin.

* Corresponding author. Mailing address: USDA/ARS/NCAUR, 1815 N. University, Peoria, IL 61604. Phone: (309) 681-6381. Fax: (309) 681-6627. E-mail: mccormsp{at}mail.ncaur.usda.gov.

dagger Present address: Novartis Agribusiness Biotechnology Research, Inc., Research Triangle Park, NC 27709.

Applied and Environmental Microbiology, December 1999, p. 5252-5256, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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