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Applied and Environmental Microbiology, December 1999, p. 5328-5333, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Listeria monocytogenes Scott A: Cell Surface Charge, Hydrophobicity, and Electron Donor and Acceptor Characteristics under Different Environmental Growth Conditions

Romain Briandet,1,2,* Thierry Meylheuc,1 Catherine Maher,3 and Marie Noëlle Bellon-Fontaine1

Institut National de la Recherche Agronomique, Unité de Recherche en Bioadhesion et Hygiène des Materiaux, Massy,1 and SKW Biosystems, Cultures & Enzymes Department, La Ferté sous Jouarre,2 France, and University College, Cork, Ireland3

Received 9 November 1998/Accepted 15 September 1999

We determined the variations in the surface physicochemical properties of Listeria monocytogenes Scott A cells that occurred under various environmental conditions. The surface charges, the hydrophobicities, and the electron donor and acceptor characteristics of L. monocytogenes Scott A cells were compared after the organism was grown in different growth media and at different temperatures; to do this, we used microelectrophoresis and the microbial adhesion to solvents method. Supplementing the growth media with glucose or lactic acid affected the electrical, hydrophobic, and electron donor and acceptor properties of the cells, whereas the growth temperature (37, 20, 15, or 8°C) primarily affected the electrical and electron donor and acceptor properties. The nonlinear effects of the growth temperature on the physicochemical properties of the cells were similar for cells cultivated in two different growth media, but bacteria cultivated in Trypticase soy broth supplemented with 6 g of yeast extract per liter (TSYE) were slightly more hydrophobic than cells cultivated in brain heart infusion medium (P < 0.05). Adhesion experiments conducted with L. monocytogenes Scott A cells cultivated in TSYE at 37, 20, 15, and 8°C and then suspended in a sodium chloride solution (1.5 × 10-1 or 1.5 × 10-3 M NaCl) confirmed that the cell surface charge and the electron donor and acceptor properties of the cells had an influence on their attachment to stainless steel.

* Corresponding author. Mailing address: INRA UBHM, 25 Avenue République, 91300 Massy, France. Phone: 33 1 69 53 64 00. Fax: 33 1 60 13 36 01. E-mail: briandet{at}massy.inra.fr.

Applied and Environmental Microbiology, December 1999, p. 5328-5333, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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