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Applied and Environmental Microbiology, December 1999, p. 5427-5430, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning, Sequencing, and Characterization of Genomic Subtracted Sequences from Listeria monocytogenes

Fone-Mao Wu and Peter M. Muriana*

Department of Food Science, Purdue University, West Lafayette, Indiana 47907

Received 18 September 1998/Accepted 13 September 1999

Individual sequences of a genomic subtracted, PCR-amplified, mixed-sequence probe (GS probe) were cloned and sequenced. The GS probe differentiated restriction fragment length polymorphism patterns for Listeria monocytogenes but did not hybridize with members of other bacterial genera. Sequence analysis identified several L. monocytogenes sequences already present in the GenBank database; the putative identities of other sequences were inferred from homology data, and still other sequences did not exhibit significant levels of homology with any GenBank sequences.

* Corresponding author. Present address: Food & Ag Products Center, Oklahoma State University, Stillwater, OK 74078-6055. Phone: (405) 744-5563. Fax: (405) 744-6313. E-mail: muriana{at}okway.okstate.edu.

Applied and Environmental Microbiology, December 1999, p. 5427-5430, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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