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Applied and Environmental Microbiology, February 1999, p. 472-476, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Genotype Analysis of Escherichia coli
Strains Isolated from Children and Chickens Living in Close
Contact
S.
Kariuki,1,2,3,*
C.
Gilks,2
J.
Kimari,3
A.
Obanda,3
J.
Muyodi,3
P.
Waiyaki,3 and
C.
A.
Hart1
Department of Medical Microbiology and
Genitourinary Medicine1 and
School
of Tropical Medicine,2 University of
Liverpool, Liverpool L69 3GA, United Kingdom, and
Centre
for Microbiology Research, Kenya Medical Research Institute,
Nairobi, Kenya3
Received 11 September 1998/Accepted 19 November 1998
Escherichia coli isolates from rectal swabs from 62 chickens and stools from 42 children living in close contact with
chickens on the same farms in Kiambu district, Kenya, were compared for their genetic relatedness. Antibiotic susceptibility profiles broadly
categorized isolates from the children and from the chickens into two
separate clusters: the majority (144; 85.5%) of the E. coli isolates from children were multidrug resistant, while the majority (216; 87.1%) of the E. coli isolates from
chickens were either fully susceptible or resistant only to
tetracycline. Sixty- and 100- to 110-MDA plasmids were found to encode
the transferable resistance to co-trimoxazole and tetracycline.
HindIII restriction endonuclease digestion of the 60- and
100- to 110-MDA plasmids produced four distinct patterns for isolates
from children and three distinct patterns for isolates from chickens.
XbaI digestion of genomic DNA followed by pulsed-field gel
electrophoresis (PFGE) analysis produced 14 distinct clusters. There
were six distinct PFGE clusters among the isolates from children, while
among the isolates from chickens there were seven distinct clusters.
Only one PFGE cluster contained isolates from both children and
chickens, with the isolates displaying an approximately 60%
coefficient of similarity. This study showed that although several
different genotypes of E. coli were isolated from
children and chickens from the same farms, the E. coli
strains from these two sources were distinct.
*
Corresponding author. Mailing address: Kenya Medical
Research Institute, Centre for Microbiology Research, P.O. Box 43640, Nairobi, Kenya. Phone: 254.2.725398. Fax: 254.2.711673. E-mail: cmr{at}insightkenya.com.
Applied and Environmental Microbiology, February 1999, p. 472-476, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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