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Applied and Environmental Microbiology, February 1999, p. 472-476, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genotype Analysis of Escherichia coli Strains Isolated from Children and Chickens Living in Close Contact

S. Kariuki,1,2,3,* C. Gilks,2 J. Kimari,3 A. Obanda,3 J. Muyodi,3 P. Waiyaki,3 and C. A. Hart1

Department of Medical Microbiology and Genitourinary Medicine1 and School of Tropical Medicine,2 University of Liverpool, Liverpool L69 3GA, United Kingdom, and Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya3

Received 11 September 1998/Accepted 19 November 1998

Escherichia coli isolates from rectal swabs from 62 chickens and stools from 42 children living in close contact with chickens on the same farms in Kiambu district, Kenya, were compared for their genetic relatedness. Antibiotic susceptibility profiles broadly categorized isolates from the children and from the chickens into two separate clusters: the majority (144; 85.5%) of the E. coli isolates from children were multidrug resistant, while the majority (216; 87.1%) of the E. coli isolates from chickens were either fully susceptible or resistant only to tetracycline. Sixty- and 100- to 110-MDA plasmids were found to encode the transferable resistance to co-trimoxazole and tetracycline. HindIII restriction endonuclease digestion of the 60- and 100- to 110-MDA plasmids produced four distinct patterns for isolates from children and three distinct patterns for isolates from chickens. XbaI digestion of genomic DNA followed by pulsed-field gel electrophoresis (PFGE) analysis produced 14 distinct clusters. There were six distinct PFGE clusters among the isolates from children, while among the isolates from chickens there were seven distinct clusters. Only one PFGE cluster contained isolates from both children and chickens, with the isolates displaying an approximately 60% coefficient of similarity. This study showed that although several different genotypes of E. coli were isolated from children and chickens from the same farms, the E. coli strains from these two sources were distinct.

* Corresponding author. Mailing address: Kenya Medical Research Institute, Centre for Microbiology Research, P.O. Box 43640, Nairobi, Kenya. Phone: 254.2.725398. Fax: 254.2.711673. E-mail: cmr{at}insightkenya.com.

Applied and Environmental Microbiology, February 1999, p. 472-476, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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