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Applied and Environmental Microbiology, February 1999, p. 648-651, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Detection of Methanotrophs in Groundwater by
PCR
Y. S.
Cheng,
J. L.
Halsey,
K. A.
Fode,
C.
C.
Remsen, and
M. L. P.
Collins*
Department of Biological Sciences and Great
Lakes Water Institute, University of Wisconsin
Milwaukee,
Milwaukee, Wisconsin 53201
Received 1 October 1998/Accepted 16 November 1998
Methanotrophic bacteria have significant potential for
bioremediation, which would require methods for monitoring the presence and activity of these organisms in environmental samples. In this study, PCR was used to detect methanotrophic bacteria. Primers were
designed on the basis of a partial sequence of pmoA, which encodes one of the proteins of the particulate methane monooxygenase. Specific amplification of a portion of pmoA was obtained
with template DNA isolated from lab strains of methanotrophs. A
pmoA product was also obtained by using DNA from
groundwater. The identity of the PCR product was confirmed by
sequencing or by amplification with a nested primer. Reverse
transcriptase PCR detected pmoA mRNA.
*
Corresponding author. Mailing address: Department of
Biological Sciences, University of Wisconsin, Milwaukee, P.O. Box 413, Milwaukee, WI 53201. Phone: (414) 229-5298. Fax: (414) 229-3926. E-mail: mlpcolli{at}uwm.edu.

This is publication no. 416 from the Center for Great Lakes
Studies.
Applied and Environmental Microbiology, February 1999, p. 648-651, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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