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Applied and Environmental Microbiology, February 1999, p. 694-697, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Properties of a Xylan-Binding Endoxylanase from Alkaliphilic Bacillus sp. Strain K-1

Khanok Ratanakhanokchai,* Khin Lay Kyu, and Morakot Tanticharoen

School of Bioresources and Technology, King Mongkut's University of Technology, Thonburi, Bangkok 10140, Thailand

Received 24 June 1998/Accepted 9 November 1998

An alkaliphilic bacterium, Bacillus sp. strain K-1, produces extracellular xylanolytic enzymes such as xylanases, beta -xylosidase, arabinofuranosidase, and acetyl esterase when grown in xylan medium. One of the extracellular xylanases that is stable in an alkaline state was purified to homogeneity by affinity adsorption-desorption on insoluble xylan. The enzyme bound to insoluble xylan but not to crystalline cellulose. The molecular mass of the purified xylan-binding xylanase was estimated to be approximately 23 kDa. The enzyme was stable at alkaline pHs up to 12. The optimum temperature and optimum pH of the enzyme activity were 60°C and 5.5, respectively. Metal ions such as Fe2+, Ca2+, and Mg2+ greatly increased the xylanase activity, whereas Mn2+ strongly inhibited it. We also demonstrated that the enzyme could hydrolyze the raw lignocellulosic substances effectively. The enzymatic products of xylan hydrolysis were a series of short-chain xylooligosaccharides, indicating that the enzyme was an endoxylanase.

* Corresponding author. Mailing address: School of Bioresources and Technology, King Mongkut's University of Technology, Thonburi, Bangkok 10140, Thailand. Phone: 662-470 9771. Fax: 662-427 9623. E-mail: ikhachai{at}cc.kmitt.ac.th.

Applied and Environmental Microbiology, February 1999, p. 694-697, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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