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Applied and Environmental Microbiology, February 1999, p. 746-751, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of a Bacterial Strain Able To Degrade Branched Nonylphenol

Tom Tanghe,1 Willem Dhooge,2 and Willy Verstraete1,*

Laboratory of Microbial Ecology, Department of Biochemical and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences,1 and Laboratory of Andrology, University Hospital,2 University of Ghent, B-9000 Ghent, Belgium

Received 9 June 1998/Accepted 2 November 1998

Conventional enrichment of microorganisms on branched nonylphenol (NP) as only carbon and energy source yielded mixed cultures able to grow on the organic compound. However, plating yielded no single colonies capable, alone or in combination with other isolates, of degrading the NP in liquid culture. Therefore, a special approach was used, referred to as "serial dilution-plate resuspension," to reduce culture complexity. In this way, one isolate, TTNP3, tentatively identified as a Sphingomonas sp., was found to be able to grow on NP in liquid culture. Remarkably, this isolate was able to be filtered through a 0.45-µm-pore-diameter filter. Moreover, isolate TTNP3 did not form visible colonies on mineral medium with NP, and it formed visible colonies on R2A agar only after a prolonged incubation of 1 week. High-performance liquid chromatography and gas chromatography-mass spectroscopy analysis of the culture media indicated that the strain starts the degradation of NP with a fission of the phenol ring and preferably uses the para isomer of NP and not the ortho isomer. No distinct accumulation of an intermediary product could be observed.

* Corresponding author. Mailing address: Laboratory of Microbial Ecology, Department of Biochemical and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, University of Ghent, Coupure Links 653, B-9000 Ghent, Belgium. Phone: 32 9 264 59 76. Fax: 32 9 264 62 48. E-mail: Willy.Verstraete{at}rug.ac.be.

Applied and Environmental Microbiology, February 1999, p. 746-751, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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