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Applied and Environmental Microbiology, February 1999, p. 834-836, Vol. 65, No. 2
0099-2240/99/$00.00+0

Green Fluorescent Protein as a Reporter To Monitor Gene Expression and Food Colonization by Aspergillus flavus

Wanglei Du,1 Zhengyu Huang,2 Joseph E. Flaherty,1 Kevin Wells,3 and Gary A. Payne1,*

Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695-76161; Dow AgrosSciences LLC, Indianapolis, Indiana 46268-10542; and USDA-ARS/Livestock and Poultry Science Institute, Gene Evaluation and Mapping Laboratory, Beltsville, Maryland 207053

Received 16 July 1998/Accepted 21 October 1998

Transformants of Aspergillus flavus containing the Aequorea victoria gfp gene fused to a viral promoter or the promoter region and 483 bp of the coding region of A. flavus aflR expressed green fluorescence detectable without a microscope or filters. Expression of green fluorescent protein fluorescence was correlated with resistance to aflatoxin accumulation in five corn genotypes inoculated with these transformants.

* Corresponding author. Mailing address: Department of Plant Pathology, Box 7616, North Carolina State University, Raleigh, NC 27695-7616. Phone: (919) 515-6994. Fax: (919) 515-7716. E-mail: gary_payne{at}ncsu.edu.

Applied and Environmental Microbiology, February 1999, p. 834-836, Vol. 65, No. 2
0099-2240/99/$00.00+0


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  • Lorang, J. M., Tuori, R. P., Martinez, J. P., Sawyer, T. L., Redman, R. S., Rollins, J. A., Wolpert, T. J., Johnson, K. B., Rodriguez, R. J., Dickman, M. B., Ciuffetti, L. M. (2001). Green Fluorescent Protein Is Lighting Up Fungal Biology. Appl. Environ. Microbiol. 67: 1987-1994 [Full Text]  


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