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Applied and Environmental Microbiology, February 1999, p. 849-852, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
New Assay for Rhizobitoxine Based on Inhibition of
1-Aminocyclopropane-1-Carboxylate Synthase
Tsuyoshi
Yasuta,1
Shigeru
Satoh,2 and
Kiwamu
Minamisawa1,*
Institute of Genetic Ecology, Tohoku
University, Katahira, Aoba-ku, Sendai
980-8577,1 and
Laboratory of
Bio-adaptation, Graduate School of Agricultural Science, Tohoku
University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai
981-8555,2 Japan
Received 9 July 1998/Accepted 12 November 1998
Rhizobitoxine is synthesized by the legume symbiont
Bradyrhizobium elkanii and the plant pathogen
Burkholderia andropogonis. Rhizobitoxine competitively
inhibited 1-aminocyclopropane-1-carboxylate (ACC) synthase bLE-ACS2
from the tomato, a key enzyme in the pathway of ethylene biosynthesis.
Based on this inhibition of ACC synthase, we have
developed a new assay for rhizobitoxine.
*
Corresponding author. Mailing address: Institute of
Genetic Ecology, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan. Phone: 81-22-217-5684. Fax: 81-22-263-9845. E-mail:
kiwamu{at}ige.tohoku.ac.jp.
Applied and Environmental Microbiology, February 1999, p. 849-852, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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