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Applied and Environmental Microbiology, February 1999, p. 873-876, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Comparative Study of Methods To Validate Formaldehyde Decontamination of Biological Safety Cabinets

Kerry Munro,1,* Janice Lanser,1 and Robert Flower2,3

Infectious Diseases Laboratories, Institute of Medical and Veterinary Science,1 and School of Pharmacy and Medical Sciences, University of South Australia,2 Adelaide, South Australia, and Pacific Laboratory Medicine Services, Royal North Shore Hospital, St. Leonards, New South Wales,3 Australia

Received 11 June 1998/Accepted 2 October 1998

Methods of validation of formaldehyde decontamination of biological safety cabinets were compared. Decontamination of metal strips inoculated with Mycobacterium bovis, poliovirus, or Bacillus spp. spores was compared with the results obtained with three biological indicators. Conditions for successful decontamination, particularly relative humidity, were defined. The Attest 1291 biological indicator was the only biological indicator which was an aid in the detection of gross decontamination failure.

* Corresponding author. Mailing address: Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Frome Rd., Adelaide, South Australia, 5000, Australia. Phone: 61 08 822 23558. Fax: 61 08 822 23543. E-mail: kerry.munro{at}imvs.sa.gov.au.

Applied and Environmental Microbiology, February 1999, p. 873-876, Vol. 65, No. 2
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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