This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayatsu, M.
Right arrow Articles by Nagata, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayatsu, M.
Right arrow Articles by Nagata, T.
Agricola
Right arrow Articles by Hayatsu, M.
Right arrow Articles by Nagata, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 1999, p. 1015-1019, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Involvement of Two Plasmids in the Degradation of Carbaryl by Arthrobacter sp. Strain RC100

Masahito Hayatsu,1,* Motoko Hirano,1 and Tadahiro Nagata2

Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529,1 and National Research Institute of Food, Tukuba, Ibaragi 305-8604,2 Japan

Received 14 September 1998/Accepted 11 December 1998

A bacterium capable of utilizing carbaryl (1-naphthyl N-methylcarbamate) as the sole carbon source was isolated from carbaryl-treated soil. This bacterium was characterized taxonomically as Arthrobacter and was designated strain RC100. RC100 hydrolyzes the N-methylcarbamate linkage to 1-naphthol, which was further metabolized via salicylate and gentisate. Strain RC100 harbored three plasmids (designated pRC1, pRC2, and pRC3). Mutants unable to degrade carbaryl arose at a high frequency after treating the culture with mitomycin C. All carbaryl-hydrolysis-deficient mutants (Cah-) lacked pRC1, and all 1-naphthol-utilization-deficient mutants (Nat-) lacked pRC2. The plasmid-free strain RC107 grew on gentisate as a carbon source. These two plasmids could be transferred to Cah- mutants or Nat- mutants by conjugation, resulting in the restoration of the Cah and Nah phenotypes.

* Corresponding author. Mailing address: Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. Phone and Fax: 81-54-238-4875. E-mail: ahmhaya{at}agr.shizuoka.ac.jp.

Applied and Environmental Microbiology, March 1999, p. 1015-1019, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shapir, N., Mongodin, E. F., Sadowsky, M. J., Daugherty, S. C., Nelson, K. E., Wackett, L. P. (2007). Evolution of Catabolic Pathways: Genomic Insights into Microbial s-Triazine Metabolism. J. Bacteriol. 189: 674-682 [Full Text]  
  • Swetha, V. P., Phale, P. S. (2005). Metabolism of Carbaryl via 1,2-Dihydroxynaphthalene by Soil Isolates Pseudomonas sp. Strains C4, C5, and C6. Appl. Environ. Microbiol. 71: 5951-5956 [Abstract] [Full Text]  
  • Overhage, J., Sielker, S., Homburg, S., Parschat, K., Fetzner, S. (2005). Identification of large linear plasmids in Arthrobacter spp. encoding the degradation of quinaldine to anthranilate. Microbiology 151: 491-500 [Abstract] [Full Text]  
  • Hashimoto, M., Fukui, M., Hayano, K., Hayatsu, M. (2002). Nucleotide Sequence and Genetic Structure of a Novel Carbaryl Hydrolase Gene (cehA) from Rhizobium sp. Strain AC100. Appl. Environ. Microbiol. 68: 1220-1227 [Abstract] [Full Text]  
  • Turnbull, G. A., Ousley, M., Walker, A., Shaw, E., Morgan, J. A. W. (2001). Degradation of Substituted Phenylurea Herbicides by Arthrobacter globiformis Strain D47 and Characterization of a Plasmid-Associated Hydrolase Gene, puhA. Appl. Environ. Microbiol. 67: 2270-2275 [Abstract] [Full Text]  
  • Hayatsu, M., Hirano, M., Tokuda, S. (2000). Involvement of Two Plasmids in Fenitrothion Degradation by Burkholderia sp. Strain NF100. Appl. Environ. Microbiol. 66: 1737-1740 [Abstract] [Full Text]  
  • Park, H.-S., Kim, H.-S. (2000). Identification and Characterization of the Nitrobenzene Catabolic Plasmids pNB1 and pNB2 in Pseudomonas putida HS12. J. Bacteriol. 182: 573-580 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»