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Applied and Environmental Microbiology, March 1999, p. 1071-1077, Vol. 65, No. 3
Nestlé Research Center, CH-1000
Lausanne 26, Switzerland,1 and CJF 94.07 INSERM, UFR de Pharmacie, Université Paris XI, F-92296
Châtenay-Malabry, France2
Received 22 May 1998/Accepted 8 December 1998
The influence of pH on the adhesion of two
Lactobacillus strains to Caco-2 human intestinal cells was
investigated. One strain, Lactobacillus johnsonii La1, was
adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same
experimental conditions. On the basis of these results, we used the
monoclonal antibody technique as a tool to determine differences on the
surface of these bacteria and to identify a factor for adhesion. Mice
were immunized with live La1, and the hybridomas produced by fusion of
spleen cells with ONS1 cells were screened for the production of
antibodies specific for L. johnsonii La1. A set of these
monoclonal antibodies was directed against a nonproteinaceous component
of the L. johnsonii La1 surface. It was identified as
lipoteichoic acid (LTA). This molecule was isolated, chemically
characterized, and tested in adhesion experiments in the same system.
The adhesion of L. johnsonii La1 to Caco-2 cells was
inhibited in a concentration-dependent way by purified LTA as well as
by L. johnsonii La1 culture supernatant that contained LTA.
These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Cell Surface-Associated Lipoteichoic Acid Acts as
an Adhesion Factor for Attachment of Lactobacillus johnsonii
La1 to Human Enterocyte-Like Caco-2 Cells
*
Corresponding author. Mailing address: Nestlé
Research Center, P.O. Box 44, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland. Phone: 41 21 785 8685. Fax: 41 21 785 89 25. E-mail:
dominique.granato{at}chlsnr.nestrd.ch.
Present address: Lactalis Research and Development, 53000 Laval, France.
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