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Applied and Environmental Microbiology, March 1999, p. 1083-1091, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Degradation of Chloronitrobenzenes by a Coculture
of Pseudomonas putida and a Rhodococcus
sp.
Hee-Sung
Park,1
Sung-Jin
Lim,2
Young Keun
Chang,1
Andrew G.
Livingston,3 and
Hak-Sung
Kim2,*
Department of Chemical
Engineering1 and Department of
Biological Sciences,2 Korea Advanced
Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu,
Taejon 305-701, Korea, and Department of Chemical
Engineering and Chemical Technology, Imperial College of Science
and Technology, London SW7 2BY, United
Kingdom3
Received 29 September 1998/Accepted 5 January 1999
A single microorganism able to mineralize chloronitrobenzenes
(CNBs) has not been reported, and degradation of CNBs by coculture of
two microbial strains was attempted. Pseudomonas putida
HS12 was first isolated by analogue enrichment culture using
nitrobenzene (NB) as the substrate, and this strain was observed to
possess a partial reductive pathway for the degradation of NB. From
high-performance liquid chromatography-mass spectrometry and
1H nuclear magnetic resonance analyses, NB-grown cells of
P. putida HS12 were found to convert 3- and 4-CNBs to the
corresponding 5- and 4-chloro-2-hydroxyacetanilides, respectively, by
partial reduction and subsequent acetylation. For the degradation of
CNBs, Rhodococcus sp. strain HS51, which
degrades 4- and 5-chloro-2-hydroxyacetanilides, was isolated and
combined with P. putida HS12 to give a coculture. This
coculture was confirmed to mineralize 3- and 4-CNBs in the presence of
an additional carbon source. A degradation pathway for 3- and 4-CNBs by
the two isolated strains was also proposed.
*
Corresponding author. Mailing address: Department of
Biological Sciences, Korea Advanced Institute of Science and
Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea. Phone:
82-42-869-2616. Fax: 82-42-869-2610. E-mail:
hskim{at}sorak.kaist.ac.kr.
Applied and Environmental Microbiology, March 1999, p. 1083-1091, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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