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Applied and Environmental Microbiology, March 1999, p. 1214-1221, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate

T. L. Kieft,1,* J. K. Fredrickson,2 T. C. Onstott,3 Y. A. Gorby,2 H. M. Kostandarithes,2 T. J. Bailey,2 D. W. Kennedy,2 S. W. Li,2 A. E. Plymale,2 C. M. Spadoni,2 and M. S. Gray2

Department of Biology, New Mexico Institute of Mining and Technology, Socorro, New Mexico 878011; Pacific Northwest National Laboratory, Richland, Washington 993522; and Department of Geosciences, Princeton University, Princeton, New Jersey 085443

Received 17 September 1998/Accepted 16 December 1998

A thermophilic bacterium that can use O2, NO3-, Fe(III), and S0 as terminal electron acceptors for growth was isolated from groundwater sampled at a 3.2-km depth in a South African gold mine. This organism, designated SA-01, clustered most closely with members of the genus Thermus, as determined by 16S rRNA gene (rDNA) sequence analysis. The 16S rDNA sequence of SA-01 was >98% similar to that of Thermus strain NMX2 A.1, which was previously isolated by other investigators from a thermal spring in New Mexico. Strain NMX2 A.1 was also able to reduce Fe(III) and other electron acceptors. Neither SA-01 nor NMX2 A.1 grew fermentatively, i.e., addition of an external electron acceptor was required for anaerobic growth. Thermus strain SA-01 reduced soluble Fe(III) complexed with citrate or nitrilotriacetic acid (NTA); however, it could reduce only relatively small quantities (0.5 mM) of hydrous ferric oxide except when the humic acid analog 2,6-anthraquinone disulfonate was added as an electron shuttle, in which case 10 mM Fe(III) was reduced. Fe(III)-NTA was reduced quantitatively to Fe(II); reduction of Fe(III)-NTA was coupled to the oxidation of lactate and supported growth through three consecutive transfers. Suspensions of Thermus strain SA-01 cells also reduced Mn(IV), Co(III)-EDTA, Cr(VI), and U(VI). Mn(IV)-oxide was reduced in the presence of either lactate or H2. Both strains were also able to mineralize NTA to CO2 and to couple its oxidation to Fe(III) reduction and growth. The optimum temperature for growth and Fe(III) reduction by Thermus strains SA-01 and NMX2 A.1 is approximately 65°C; their optimum pH is 6.5 to 7.0. This is the first report of a Thermus sp. being able to couple the oxidation of organic compounds to the reduction of Fe, Mn, or S.

* Corresponding author. Mailing address: Department of Biology, New Mexico Institute of Mining and Technology, Socorro, NM 87801. Phone: (505) 835-5321. Fax: (505) 835-6329. E-mail: tkieft{at}nmt.edu.

Applied and Environmental Microbiology, March 1999, p. 1214-1221, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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