A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Daffonchio, D.
	Articles by Sorlini, C.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Daffonchio, D.
	Articles by Sorlini, C.


Agricola

	Articles by Daffonchio, D.
	Articles by Sorlini, C.

Applied and Environmental Microbiology, March 1999, p. 1298-1303, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis

Daniele Daffonchio,¹^,^* Sara Borin,¹ Giuseppe Frova,¹ Romina Gallo,² Elena Mori,² Renato Fani,² and Claudia Sorlini¹

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan,¹ and Dipartimento di Biologia Animale e Genetica, Università degli Studi, 50125 Florence,² Italy

Received 22 September 1998/Accepted 3 December 1998

Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus,Bacillus thuringiensis, and Bacillus mycoides, we applied therandomly amplified polymorphic DNA (RAPD) fingerprinting techniqueto a collection of 101 strains of the genus Bacillus, including61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850)specific for B. cereus, B. thuringiensis, B. anthracis, and B.mycoides was identified. This fragment included a putative (366-nucleotide)open reading frame highly homologous to the ypuA gene of Bacillussubtilis. The restriction analysis of the SG-850 fragment withAluI distinguished B. anthracis from the other species of theB. cereusgroup.

^* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degliStudi di Milano, via Celoria 2, 20133 Milan, Italy. Phone: 39-02-23955845.Fax: 39-02-70630829. E-mail: fonchius{at}imiucca.csi.unimi.it.

Applied and Environmental Microbiology, March 1999, p. 1298-1303, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Kim, W., Kim, J.-Y., Cho, S.-L., Nam, S.-W., Shin, J.-W., Kim, Y.-S., Shin, H.-S. (2008). Glycosyltransferase - a specific marker for the discrimination of Bacillus anthracis from the Bacillus cereus group. J Med Microbiol 57: 279-286 [Abstract] [Full Text]
Merabishvili, M., Natidze, M., Rigvava, S., Brusetti, L., Raddadi, N., Borin, S., Chanishvili, N., Tediashvili, M., Sharp, R., Barbeschi, M., Visca, P., Daffonchio, D. (2006). Diversity of Bacillus anthracis Strains in Georgia and of Vaccine Strains from the Former Soviet Union. Appl. Environ. Microbiol. 72: 5631-5636 [Abstract] [Full Text]
Klee, S. R., Ozel, M., Appel, B., Boesch, C., Ellerbrok, H., Jacob, D., Holland, G., Leendertz, F. H., Pauli, G., Grunow, R., Nattermann, H. (2006). Characterization of Bacillus anthracis-Like Bacteria Isolated from Wild Great Apes from Cote d'Ivoire and Cameroon.. J. Bacteriol. 188: 5333-5344 [Abstract] [Full Text]
Zasada, A. A., Gierczynski, R., Raddadi, N., Daffonchio, D., Jagielski, M. (2006). Some Bacillus thuringiensis Strains Share rpoB Nucleotide Polymorphisms Also Present in Bacillus anthracis. J. Clin. Microbiol. 44: 1606-1607 [Full Text]
Daffonchio, D., Raddadi, N., Merabishvili, M., Cherif, A., Carmagnola, L., Brusetti, L., Rizzi, A., Chanishvili, N., Visca, P., Sharp, R., Borin, S. (2006). Strategy for Identification of Bacillus cereus and Bacillus thuringiensis Strains Closely Related to Bacillus anthracis. Appl. Environ. Microbiol. 72: 1295-1301 [Abstract] [Full Text]
Valjevac, S., Hilaire, V., Lisanti, O., Ramisse, F., Hernandez, E., Cavallo, J.-D., Pourcel, C., Vergnaud, G. (2005). Comparison of Minisatellite Polymorphisms in the Bacillus cereus Complex: a Simple Assay for Large-Scale Screening and Identification of Strains Most Closely Related to Bacillus anthracis. Appl. Environ. Microbiol. 71: 6613-6623 [Abstract] [Full Text]
Ryu, C., Lee, K., Hawng, H.-J., Yoo, C.-K., Seong, W.-K., Oh, H.-B. (2005). Molecular Characterization of Korean Bacillus anthracis Isolates by Amplified Fragment Length Polymorphism Analysis and Multilocus Variable-Number Tandem Repeat Analysis. Appl. Environ. Microbiol. 71: 4664-4671 [Abstract] [Full Text]
Ehling-Schulz, M., Svensson, B., Guinebretiere, M.-H., Lindback, T., Andersson, M., Schulz, A., Fricker, M., Christiansson, A., Granum, P. E., Martlbauer, E., Nguyen-The, C., Salkinoja-Salonen, M., Scherer, S. (2005). Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology 151: 183-197 [Abstract] [Full Text]
Bode, E., Hurtle, W., Norwood, D. (2004). Real-Time PCR Assay for a Unique Chromosomal Sequence of Bacillus anthracis. J. Clin. Microbiol. 42: 5825-5831 [Abstract] [Full Text]
Hurtle, W., Bode, E., Kulesh, D. A., Kaplan, R. S., Garrison, J., Bridge, D., House, M., Frye, M. S., Loveless, B., Norwood, D. (2004). Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe. J. Clin. Microbiol. 42: 179-185 [Abstract] [Full Text]
Ko, K. S., Kim, J.-M., Kim, J.-W., Jung, B. Y., Kim, W., Kim, I. J., Kook, Y.-H. (2003). Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR. J. Clin. Microbiol. 41: 2908-2914 [Abstract] [Full Text]
Radnedge, L., Agron, P. G., Hill, K. K., Jackson, P. J., Ticknor, L. O., Keim, P., Andersen, G. L. (2003). Genome Differences That Distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 69: 2755-2764 [Abstract] [Full Text]
Luna, V. A., King, D., Davis, C., Rycerz, T., Ewert, M., Cannons, A., Amuso, P., Cattani, J. (2003). Novel Sample Preparation Method for Safe and Rapid Detection of Bacillus anthracis Spores in Environmental Powders and Nasal Swabs. J. Clin. Microbiol. 41: 1252-1255 [Abstract] [Full Text]
Becker, K., Badehorn, D., Keller, B., Schulte, M., Bohm, K. H., Peters, G., Fegeler, W. (2001). Isolation and Characterization of a Species-Specific DNA Fragment for Identification of Candida (Torulopsis) glabrata by PCR. J. Clin. Microbiol. 39: 3356-3359 [Abstract] [Full Text]
Qi, Y., Patra, G., Liang, X., Williams, L. E., Rose, S., Redkar, R. J., DelVecchio, V. G. (2001). Utilization of the rpoB Gene as a Specific Chromosomal Marker for Real-Time PCR Detection of Bacillus anthracis. Appl. Environ. Microbiol. 67: 3720-3727 [Abstract] [Full Text]
Senesi, S., Celandroni, F., Tavanti, A., Ghelardi, E. (2001). Molecular Characterization and Identification of Bacillus clausii Strains Marketed for Use in Oral Bacteriotherapy. Appl. Environ. Microbiol. 67: 834-839 [Abstract] [Full Text]
Daffonchio, D., Cherif, A., Borin, S. (2000). Homoduplex and Heteroduplex Polymorphisms of the Amplified Ribosomal 16S-23S Internal Transcribed Spacers Describe Genetic Relationships in the "Bacillus cereus Group". Appl. Environ. Microbiol. 66: 5460-5468 [Abstract] [Full Text]
Prüß, B. M., Dietrich, R., Nibler, B., Märtlbauer, E., Scherer, S. (1999). The Hemolytic Enterotoxin HBL Is Broadly Distributed among Species of the Bacillus cereus Group. Appl. Environ. Microbiol. 65: 5436-5442 [Abstract] [Full Text]

J. Bacteriol.	Microbiol. Mol. Biol. Rev.	Eukaryot. Cell	All ASM Journals