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Applied and Environmental Microbiology, March 1999, p. 1320-1324, Vol. 65, No. 3
0099-2240/99

Inhibition of Plant-Pathogenic Fungi by a Corn Trypsin Inhibitor Overexpressed in Escherichia coli

Zhi-Yuan Chen,1 Robert L. Brown,2,* Alan R. Lax,2 Thomas E. Cleveland,2 and John S. Russin1

Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803,1 and Southern Regional Research Center, USDA Agricultural Research Service, New Orleans, Louisiana 701792

Received 10 August 1998/Accepted 30 November 1998

The cDNA of a 14-kDa trypsin inhibitor (TI) from corn was subcloned into an Escherichia coli overexpression vector. The overexpressed TI was purified based on its insolubility in urea and then refolded into the active form in vitro. This recombinant TI inhibited both conidium germination and hyphal growth of all nine plant pathogenic fungi studied, including Aspergillus flavus, Aspergillus parasiticus, and Fusarium moniliforme. The calculated 50% inhibitory concentration of TI for conidium germination ranged from 70 to more than 300 µg/ml, and that for fungal growth ranged from 33 to 124 µg/ml depending on the fungal species. It also inhibited A. flavus and F. moniliforme simultaneously when they were tested together. The results suggest that the corn 14-kDa TI may function in host resistance against a variety of fungal pathogens of crops.

* Corresponding author. Mailing address: USDA/ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70179. Phone: (504) 286-4359. Fax: (504) 286-4419. E-mail: rbrown{at}nola.srrc.usda.gov.

Applied and Environmental Microbiology, March 1999, p. 1320-1324, Vol. 65, No. 3
0099-2240/99


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