Previous Article | Next Article 
Applied and Environmental Microbiology, March 1999, p. 969-973, Vol. 65, No. 3
Danish Institute for Fisheries Research,
Received 29 September 1998/Accepted 22 December 1998
To study the possible use of probiotics in fish farming, we
evaluated the in vitro and in vivo antagonism of antibacterial strain
Pseudomonas fluorescens strain AH2 against the
fish-pathogenic bacterium Vibrio anguillarum. As iron is
important in virulence and bacterial interactions, the effect of
P. fluorescens AH2 was studied under iron-rich and
iron-limited conditions. Sterile-filtered culture supernatants from
iron-limited P. fluorescens AH2 inhibited the growth of
V. anguillarum, whereas sterile-filtered supernatants from
iron-replete cultures of P. fluorescens AH2 did not.
P. fluorescens AH2 inhibited the growth of V. anguillarum during coculture, independently of the iron
concentration, when the initial count of the antagonist was 100 to
1,000 times greater that of the fish pathogen. These in vitro results
were successfully repeated in vivo. A probiotic effect in vivo was
tested by exposing rainbow trout (Oncorynchus mykiss
Walbaum) to P. fluorescens AH2 at a density of
105 CFU/ml for 5 days before a challenge with V. anguillarum at 104 to 105 CFU/ml for
1 h. Some fish were also exposed to P. fluorescens AH2
at 107 CFU/ml during the 1-h infection. The combined
probiotic treatment resulted in a 46% reduction of calculated
accumulated mortality; accumulated mortality was 25% after 7 days at
12°C in the probiotic-treated fish, whereas mortality was 47% in
fish not treated with the probiont.
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Inhibition of Vibrio anguillarum by
Pseudomonas fluorescens AH2, a Possible Probiotic Treatment
of Fish
*
Corresponding author. Mailing address: Danish Institute
for Fisheries Research, Department of Seafood Research, Technical University of Denmark, Bldg. 221, DK-2800 Lyngby, Denmark. Phone: 45 4525 2586. Fax: 45 4588 4774. E-mail: gram{at}dfu.min.dk.
Applied and Environmental Microbiology, March 1999, p. 969-973, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Vejborg, R. M., Klemm, P.
(2008). Blocking of Bacterial Biofilm Formation by a Fish Protein Coating. Appl. Environ. Microbiol.
74: 3551-3558
[Abstract] [Full Text] -
Bruhn, J. B., Nielsen, K. F., Hjelm, M., Hansen, M., Bresciani, J., Schulz, S., Gram, L.
(2005). Ecology, Inhibitory Activity, and Morphogenesis of a Marine Antagonistic Bacterium Belonging to the Roseobacter Clade. Appl. Environ. Microbiol.
71: 7263-7270
[Abstract] [Full Text] -
Hjelm, M., Riaza, A., Formoso, F., Melchiorsen, J., Gram, L.
(2004). Seasonal Incidence of Autochthonous Antagonistic Roseobacter spp. and Vibrionaceae Strains in a Turbot Larva (Scophthalmus maximus) Rearing System. Appl. Environ. Microbiol.
70: 7288-7294
[Abstract] [Full Text] -
Holmstrom, K., Gram, L.
(2003). Elucidation of the Vibrio anguillarum Genetic Response to the Potential Fish Probiont Pseudomonas fluorescens AH2, Using RNA-Arbitrarily Primed PCR. J. Bacteriol.
185: 831-842
[Abstract] [Full Text] -
Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W.
(2000). Probiotic Bacteria as Biological Control Agents in Aquaculture. Microbiol. Mol. Biol. Rev.
64: 655-671
[Abstract] [Full Text] -
Verschuere, L., Heang, H., Criel, G., Sorgeloos, P., Verstraete, W.
(2000). Selected Bacterial Strains Protect Artemia spp. from the Pathogenic Effects of Vibrio proteolyticus CW8T2. Appl. Environ. Microbiol.
66: 1139-1146
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»