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Applied and Environmental Microbiology, April 1999, p. 1397-1404, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development and Characterization of a Fluorescent-Bacteriophage Assay for Detection of Escherichia coli O157:H7

Lawrence Goodridge, Jinru Chen,dagger and Mansel Griffiths*

Department of Food Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1

Received 2 June 1998/Accepted 9 November 1998

In this paper we describe evaluation and characterization of a novel assay that combines immunomagnetic separation and a fluorescently stained bacteriophage for detection of Escherichia coli O157:H7 in broth. When it was combined with flow cytometry, the fluorescent-bacteriophage assay (FBA) was capable of detecting 104 cells/ml. A modified direct epifluorescent-filter technique (DEFT) was employed in an attempt to estimate bacterial concentrations. Using regression analysis, we calculated that the lower detection limit was between 102 and 103 cells/ml; however, the modified DEFT was found to be an unreliable method for determining bacterial concentrations. The results of this study show that the FBA, when combined with flow cytometry, is a sensitive technique for presumptive detection of E. coli O157:H7 in broth cultures.

* Corresponding author. Mailing address: Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: (519) 824-4120, ext. 2269. Fax: (519) 824-6631. E-mail: mgriffit{at}uoguelph.ca.

dagger Present address: Center for Food Safety & Quality Enhancement, University of Georgia, Griffin, GA 30223-1797.

Applied and Environmental Microbiology, April 1999, p. 1397-1404, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Goodridge, L., Gallaccio, A., Griffiths, M. W. (2003). Morphological, Host Range, and Genetic Characterization of Two Coliphages. Appl. Environ. Microbiol. 69: 5364-5371 [Abstract] [Full Text]  


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