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Applied and Environmental Microbiology, April 1999, p. 1450-1454, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Effectiveness of Chemometric Techniques in Discrimination of Lactobacillus helveticus Biotypes from Natural Dairy Starter Cultures on the Basis of Phenotypic Characteristics

Monica Gatti, Giovanna Contarini, and Erasmo Neviani*

Istituto Sperimentale Lattiero Caseario, 26900 Lodi, Italy

Received 29 September 1998/Accepted 26 January 1999

Lactobacillus helveticus is the dominant organism in natural starter cultures used for the production of typical Italian cheeses. In this study, 74 L. helveticus strains, isolated from grana and provolone cheese natural whey starters, were distinguished with respect to their origin by using both cell wall protein profiles and chemometric evaluation of some phenotypic parameters, such as the ability to acidify cultures and the presence of nonspecific proteolytic and peptidase activities. Cell wall protein patterns allowed L. helveticus strains to be distinguished with respect to their source of isolation. Among the different phenotypes studied, no single specific parameter permitted the two groups of strains to be separated. A good discrimination between the two groups of L. helveticus species was obtained by multivariate statistical techniques, which permitted the extraction of all of the discriminating information retained in the phenotypic activities. Associations between strain phenotype expression and dairy environmental ecosystem source are discussed.

* Corresponding author. Mailing address: Microbiology and Enzymology Department, Istituto Sperimentale Lattiero Caseario, Via A. Lombardo, 11, 26900 Lodi, Italy. Phone: 39 0371 45011. Fax: 39 0371 35579. E-mail: ilclodi{at}pop.telware.it.

Applied and Environmental Microbiology, April 1999, p. 1450-1454, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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