This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scala, D. J.
Right arrow Articles by Kerkhof, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scala, D. J.
Right arrow Articles by Kerkhof, L. J.
Agricola
Right arrow Articles by Scala, D. J.
Right arrow Articles by Kerkhof, L. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 1999, p. 1681-1687, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Diversity of Nitrous Oxide Reductase (nosZ) Genes in Continental Shelf Sediments

David J. Scala and Lee J. Kerkhof*

Institute of Marine and Coastal Sciences, Cook College, Rutgers University, New Brunswick, New Jersey 08901

Received 15 October 1998/Accepted 3 February 1999

Diversity of the nitrous oxide reductase (nosZ) gene was examined in sediments obtained from the Atlantic Ocean and Pacific Ocean continental shelves. Approximately 1,100 bp of the nosZ gene were amplified via PCR, using nosZ gene-specific primers. Thirty-seven unique copies of the nosZ gene from these marine environments were characterized, increasing the nosZ sequence database fourfold. The average DNA similarity for comparisons between all 49 variants of the nosZ gene was 64% ± 10%. Alignment of the derived amino acid sequences confirmed the conservation of important structural motifs. A highly conserved region is proposed as the copper binding, catalytic site (CuZ) of the mature protein. Phylogenetic analysis demonstrated three major clusters of nosZ genes, with little overlap between environmental and culture-based groups. Finally, the two non-culture-based gene clusters generally corresponded to sampling location, implying that denitrifier communities may be restricted geographically.

* Corresponding author. Mailing address: Institute of Marine and Coastal Sciences, Cook Campus, Rutgers University, 71 Dudley Rd., New Brunswick, NJ 08901-8521. Phone: (732) 932-6555 x335. Fax: (732) 932-6520. E-mail: kerkhof{at}ahab.rutgers.edu.

Applied and Environmental Microbiology, April 1999, p. 1681-1687, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wust, P. K., Horn, M. A., Henderson, G., Janssen, P. H., Rehm, B. H. A., Drake, H. L. (2009). Gut-Associated Denitrification and In Vivo Emission of Nitrous Oxide by the Earthworm Families Megascolecidae and Lumbricidae in New Zealand. Appl. Environ. Microbiol. 75: 3430-3436 [Abstract] [Full Text]  
  • Mills, H. J., Hunter, E., Humphrys, M., Kerkhof, L., McGuinness, L., Huettel, M., Kostka, J. E. (2008). Characterization of Nitrifying, Denitrifying, and Overall Bacterial Communities in Permeable Marine Sediments of the Northeastern Gulf of Mexico. Appl. Environ. Microbiol. 74: 4440-4453 [Abstract] [Full Text]  
  • Quemeneur, M., Heinrich-Salmeron, A., Muller, D., Lievremont, D., Jauzein, M., Bertin, P. N., Garrido, F., Joulian, C. (2008). Diversity Surveys and Evolutionary Relationships of aoxB Genes in Aerobic Arsenite-Oxidizing Bacteria. Appl. Environ. Microbiol. 74: 4567-4573 [Abstract] [Full Text]  
  • Hunter, E. M., Mills, H. J., Kostka, J. E. (2006). Microbial Community Diversity Associated with Carbon and Nitrogen Cycling in Permeable Shelf Sediments. Appl. Environ. Microbiol. 72: 5689-5701 [Abstract] [Full Text]  
  • Lalucat, J., Bennasar, A., Bosch, R., Garcia-Valdes, E., Palleroni, N. J. (2006). Biology of Pseudomonas stutzeri. Microbiol. Mol. Biol. Rev. 70: 510-547 [Abstract] [Full Text]  
  • Horn, M. A., Drake, H. L., Schramm, A. (2006). Nitrous Oxide Reductase Genes (nosZ) of Denitrifying Microbial Populations in Soil and the Earthworm Gut Are Phylogenetically Similar. Appl. Environ. Microbiol. 72: 1019-1026 [Abstract] [Full Text]  
  • Wawrik, B., Kerkhof, L., Zylstra, G. J., Kukor, J. J. (2005). Identification of Unique Type II Polyketide Synthase Genes in Soil. Appl. Environ. Microbiol. 71: 2232-2238 [Abstract] [Full Text]  
  • Yoshie, S., Noda, N., Tsuneda, S., Hirata, A., Inamori, Y. (2004). Salinity Decreases Nitrite Reductase Gene Diversity in Denitrifying Bacteria of Wastewater Treatment Systems. Appl. Environ. Microbiol. 70: 3152-3157 [Abstract] [Full Text]  
  • Fennell, D. E., Rhee, S.-K., Ahn, Y.-B., Haggblom, M. M., Kerkhof, L. J. (2004). Detection and Characterization of a Dehalogenating Microorganism by Terminal Restriction Fragment Length Polymorphism Fingerprinting of 16S rRNA in a Sulfidogenic, 2-Bromophenol-Utilizing Enrichment. Appl. Environ. Microbiol. 70: 1169-1175 [Abstract] [Full Text]  
  • Stres, B., Mahne, I., Avgustin, G., Tiedje, J. M. (2004). Nitrous Oxide Reductase (nosZ) Gene Fragments Differ between Native and Cultivated Michigan Soils. Appl. Environ. Microbiol. 70: 301-309 [Abstract] [Full Text]  
  • Rich, J. J., Heichen, R. S., Bottomley, P. J., Cromack, K. Jr., Myrold, D. D. (2003). Community Composition and Functioning of Denitrifying Bacteria from Adjacent Meadow and Forest Soils. Appl. Environ. Microbiol. 69: 5974-5982 [Abstract] [Full Text]  
  • Liu, X., Bagwell, C. E., Wu, L., Devol, A. H., Zhou, J. (2003). Molecular Diversity of Sulfate-Reducing Bacteria from Two Different Continental Margin Habitats. Appl. Environ. Microbiol. 69: 6073-6081 [Abstract] [Full Text]  
  • Bent, S. J., Gucker, C. L., Oda, Y., Forney, L. J. (2003). Spatial Distribution of Rhodopseudomonas palustris Ecotypes on a Local Scale. Appl. Environ. Microbiol. 69: 5192-5197 [Abstract] [Full Text]  
  • Braker, G., Tiedje, J. M. (2003). Nitric Oxide Reductase (norB) Genes from Pure Cultures and Environmental Samples. Appl. Environ. Microbiol. 69: 3476-3483 [Abstract] [Full Text]  
  • Liu, X., Tiquia, S. M., Holguin, G., Wu, L., Nold, S. C., Devol, A. H., Luo, K., Palumbo, A. V., Tiedje, J. M., Zhou, J. (2003). Molecular Diversity of Denitrifying Genes in Continental Margin Sediments within the Oxygen-Deficient Zone off the Pacific Coast of Mexico. Appl. Environ. Microbiol. 69: 3549-3560 [Abstract] [Full Text]  
  • Gregory, L. G., Bond, P. L., Richardson, D. J., Spiro, S. (2003). Characterization of a nitrate-respiring bacterial community using the nitrate reductase gene (narG) as a functional marker. Microbiology 149: 229-237 [Abstract] [Full Text]  
  • Nogales, B., Timmis, K. N., Nedwell, D. B., Osborn, A. M. (2002). Detection and Diversity of Expressed Denitrification Genes in Estuarine Sediments after Reverse Transcription-PCR Amplification from mRNA. Appl. Environ. Microbiol. 68: 5017-5025 [Abstract] [Full Text]  
  • Rosch, C., Mergel, A., Bothe, H. (2002). Biodiversity of Denitrifying and Dinitrogen-Fixing Bacteria in an Acid Forest Soil. Appl. Environ. Microbiol. 68: 3818-3829 [Abstract] [Full Text]  
  • Sakano, Y., Pickering, K. D., Strom, P. F., Kerkhof, L. J. (2002). Spatial Distribution of Total, Ammonia-Oxidizing, and Denitrifying Bacteria in Biological Wastewater Treatment Reactors for Bioregenerative Life Support. Appl. Environ. Microbiol. 68: 2285-2293 [Abstract] [Full Text]  
  • Prieme, A., Braker, G., Tiedje, J. M. (2002). Diversity of Nitrite Reductase (nirK and nirS) Gene Fragments in Forested Upland and Wetland Soils. Appl. Environ. Microbiol. 68: 1893-1900 [Abstract] [Full Text]  
  • Buchan, A., Neidle, E. L., Moran, M. A. (2001). Diversity of the Ring-Cleaving Dioxygenase Gene pcaH in a Salt Marsh Bacterial Community. Appl. Environ. Microbiol. 67: 5801-5809 [Abstract] [Full Text]  
  • Allen, A. E., Booth, M. G., Frischer, M. E., Verity, P. G., Zehr, J. P., Zani, S. (2001). Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria. Appl. Environ. Microbiol. 67: 5343-5348 [Abstract] [Full Text]  
  • Francis, C. A., Tebo, B. M. (2001). cumA Multicopper Oxidase Genes from Diverse Mn(II)-Oxidizing and Non-Mn(II)-Oxidizing Pseudomonas Strains. Appl. Environ. Microbiol. 67: 4272-4278 [Abstract] [Full Text]  
  • Grüntzig, V., Nold, S. C., Zhou, J., Tiedje, J. M. (2001). Pseudomonas stutzeri Nitrite Reductase Gene Abundance in Environmental Samples Measured by Real-Time PCR. Appl. Environ. Microbiol. 67: 760-768 [Abstract] [Full Text]  
  • Scala, D. J., Kerkhof, L. J. (2000). Horizontal Heterogeneity of Denitrifying Bacterial Communities in Marine Sediments by Terminal Restriction Fragment Length Polymorphism Analysis. Appl. Environ. Microbiol. 66: 1980-1986 [Abstract] [Full Text]  
  • Braker, G., Zhou, J., Wu, L., Devol, A. H., Tiedje, J. M. (2000). Nitrite Reductase Genes (nirK and nirS) as Functional Markers To Investigate Diversity of Denitrifying Bacteria in Pacific Northwest Marine Sediment Communities. Appl. Environ. Microbiol. 66: 2096-2104 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»