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Applied and Environmental Microbiology, April 1999, p. 1769-1771, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Green Fluorescent Protein To Detect Expression of an Endopolygalacturonase Gene of Colletotrichum lindemuthianum during Bean Infection

Bernard Dumas,* Sylvie Centis, Nathalie Sarrazin, and Marie-Thérèse Esquerré-Tugayé

UMR 5546 CNRS-UPS, "Signaux et Messages Cellulaires chez les Végétaux," Pôle de Biotechnologie Végétale, Auzeville 31326, Castanet-Tolosan, France

Received 16 November 1998/Accepted 26 January 1999

The 5' noncoding region of clpg2, an endopolygalacturonase gene of the bean pathogen Colletotrichum lindemuthianum, was fused to the coding sequence of a gene encoding a green fluorescent protein (GFP), and the construct was introduced into the fungal genome. Detection of GFP accumulation by fluorescence microscopy examination revealed that clpg2 was expressed at the early stages of germination of the conidia and during appressorium formation both in vitro and on the host plant.

* Corresponding author. Mailing address: UMR 5546 CNRS-UPS, "Signaux et Messages Cellulaires chez les Végétaux," Pôle de Biotechnologie Végétale, 24 Chemin de Borde-Rouge, BP17, Auzeville 31326, Castanet-Tolosan, France. Phone: (33) (0) 5-62-19-35-03. Fax: (33) (0) 5-62-19-35-25. E-mail: dumas{at}cict.fr.

Applied and Environmental Microbiology, April 1999, p. 1769-1771, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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