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Applied and Environmental Microbiology, April 1999, p. 1786-1788, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Quantitative Selective PCR of 16S Ribosomal DNA Correlates Well with Selective Agar Plating in Describing Population Dynamics of Indigenous Pseudomonas spp. in Soil Hot Spots

Kaare Johnsen,1,2,* Øivind Enger,2 Carsten S. Jacobsen,1 Laila Thirup,1 and Vigdis Torsvik2

Geological Survey of Denmark and Greenland, Copenhagen, Denmark,1 and Department of Microbiology, University of Bergen, Bergen, Norway2

Received 24 August 1998/Accepted 7 February 1999

We used a quantitative PCR method targeting 16S ribosomal DNA using competitive PCR for specific detection of indigenous Pseudomonas DNA in soil hot spots. The amount of Pseudomonas DNA corresponded to the number of culturable Pseudomonas bacteria on Gould's S1 agar. This represents the first use of PCR for quantification of indigenous bacteria in more than one sample of soil.

* Corresponding author. Mailing address: Geological Survey of Denmark and Greenland, Thoravej 8, DK-2400 Copenhagen NV, Denmark. Phone: 45 38 14 20 00. Fax: 45 38 14 20 50. E-mail: kj{at}geus.dk.

Applied and Environmental Microbiology, April 1999, p. 1786-1788, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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