This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Finlay, W. J. J.
Right arrow Articles by Sutherland, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Finlay, W. J. J.
Right arrow Articles by Sutherland, A. D.
Agricola
Right arrow Articles by Finlay, W. J. J.
Right arrow Articles by Sutherland, A. D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 1999, p. 1811-1812, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Semiautomated Metabolic Staining Assay for Bacillus cereus Emetic Toxin

W. J. J. Finlay, N. A. Logan,* and A. D. Sutherland

School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 OBA, United Kingdom

Received 14 September 1998/Accepted 18 January 1999

This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.

* Corresponding author. Mailing address: School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Rd., Glasgow G4 OBA, United Kingdom. Phone: (44) 141 331 3207. Fax: (44) 141 331 3208/3242. E-mail: N.A.Logan{at}gcal.ac.uk.

Applied and Environmental Microbiology, April 1999, p. 1811-1812, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lucking, G., Dommel, M. K., Scherer, S., Fouet, A., Ehling-Schulz, M. (2009). Cereulide synthesis in emetic Bacillus cereus is controlled by the transition state regulator AbrB, but not by the virulence regulator PlcR. Microbiology 155: 922-931 [Abstract] [Full Text]  
  • Wijnands, L. M., Dufrenne, J. B., van Leusden, F. M., Abee, T. (2007). Germination of Bacillus cereus Spores Is Induced by Germinants from Differentiated Caco-2 Cells, a Human Cell Line Mimicking the Epithelial Cells of the Small Intestine. Appl. Environ. Microbiol. 73: 5052-5054 [Abstract] [Full Text]  
  • Fricker, M., Messelhausser, U., Busch, U., Scherer, S., Ehling-Schulz, M. (2007). Diagnostic Real-Time PCR Assays for the Detection of Emetic Bacillus cereus Strains in Foods and Recent Food-Borne Outbreaks. Appl. Environ. Microbiol. 73: 1892-1898 [Abstract] [Full Text]  
  • Gray, K. M., Banada, P. P., O'Neal, E., Bhunia, A. K. (2005). Rapid Ped-2E9 Cell-Based Cytotoxicity Analysis and Genotyping of Bacillus Species. J. Clin. Microbiol. 43: 5865-5872 [Abstract] [Full Text]  
  • Dierick, K., Van Coillie, E., Swiecicka, I., Meyfroidt, G., Devlieger, H., Meulemans, A., Hoedemaekers, G., Fourie, L., Heyndrickx, M., Mahillon, J. (2005). Fatal Family Outbreak of Bacillus cereus-Associated Food Poisoning. J. Clin. Microbiol. 43: 4277-4279 [Abstract] [Full Text]  
  • Ehling-Schulz, M., Vukov, N., Schulz, A., Shaheen, R., Andersson, M., Martlbauer, E., Scherer, S. (2005). Identification and Partial Characterization of the Nonribosomal Peptide Synthetase Gene Responsible for Cereulide Production in Emetic Bacillus cereus. Appl. Environ. Microbiol. 71: 105-113 [Abstract] [Full Text]  
  • Ehling-Schulz, M., Svensson, B., Guinebretiere, M.-H., Lindback, T., Andersson, M., Schulz, A., Fricker, M., Christiansson, A., Granum, P. E., Martlbauer, E., Nguyen-The, C., Salkinoja-Salonen, M., Scherer, S. (2005). Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology 151: 183-197 [Abstract] [Full Text]  
  • Rowan, N. J., Caldow, G., Gemmell, C. G., Hunter, I. S. (2003). Production of Diarrheal Enterotoxins and Other Potential Virulence Factors by Veterinary Isolates of Bacillus Species Associated with Nongastrointestinal Infections. Appl. Environ. Microbiol. 69: 2372-2376 [Abstract] [Full Text]  
  • Haggblom, M. M, Apetroaie, C., Andersson, M. A., Salkinoja-Salonen, M. S. (2002). Quantitative Analysis of Cereulide, the Emetic Toxin of Bacillus cereus, Produced under Various Conditions. Appl. Environ. Microbiol. 68: 2479-2483 [Abstract] [Full Text]  
  • Rowan, N. J., Deans, K., Anderson, J. G., Gemmell, C. G., Hunter, I. S., Chaithong, T. (2001). Putative Virulence Factor Expression by Clinical and Food Isolates of Bacillus spp. after Growth in Reconstituted Infant Milk Formulae. Appl. Environ. Microbiol. 67: 3873-3881 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»