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Applied and Environmental Microbiology, April 1999, p. 1811-1812, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Semiautomated Metabolic Staining Assay for Bacillus cereus Emetic Toxin

W. J. J. Finlay, N. A. Logan,* and A. D. Sutherland

School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 OBA, United Kingdom

Received 14 September 1998/Accepted 18 January 1999

This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.

* Corresponding author. Mailing address: School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Rd., Glasgow G4 OBA, United Kingdom. Phone: (44) 141 331 3207. Fax: (44) 141 331 3208/3242. E-mail: N.A.Logan{at}gcal.ac.uk.

Applied and Environmental Microbiology, April 1999, p. 1811-1812, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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