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Applied and Environmental Microbiology, May 1999, p. 1876-1882, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Activation and Inactivation of Pseudomonas stutzeri Methylbenzene Catabolism Pathways Mediated by a Transposable Element

Fabrizio Bolognese, Cinzia di Lecce, Enrica Galli, and Paola Barbieri*

Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, 20133 Milan, Italy

Received 5 November 1998/Accepted 23 February 1999

The arrangement of the genes involved in o-xylene, m-xylene, and p-xylene catabolism was investigated in three Pseudomonas stutzeri strains: the wild-type strain OX1, which is able to grow on o-xylene but not on the meta and para isomers; the mutant M1, which grows on m-xylene and p-xylene but is unable to utilize the ortho isomer; and the revertant R1, which can utilize all the three isomers of xylene. A 3-kb insertion sequence (IS) termed ISPs1, which inactivates the m-xylene and p-xylene catabolic pathway in P. stutzeri OX1 and the o-xylene catabolic genes in P. stutzeri M1, was detected. No IS was detected in the corresponding catabolic regions of the P. stutzeri R1 genome. ISPs1 is present in several copies in the genomes of the three strains. It is flanked by 24-bp imperfect inverted repeats, causes the direct duplication of 8 bp in the target DNA, and seems to be related to the ISL3 family.

* Corresponding author. Mailing address: Dip. di Genetica e di Biologia dei Microrganismi, Via Celoria, 26, 20133 Milano, Italy. Phone: (39) 02.266.05.227. Fax: (39) 02.266.45.51. E-mail: Barbieri{at}imiucca.csi.unimi.it.

Applied and Environmental Microbiology, May 1999, p. 1876-1882, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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