This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bultreys, A.
Right arrow Articles by Gheysen, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bultreys, A.
Right arrow Articles by Gheysen, I.
Agricola
Right arrow Articles by Bultreys, A.
Right arrow Articles by Gheysen, I.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 1999, p. 1904-1909, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Biological and Molecular Detection of Toxic Lipodepsipeptide-Producing Pseudomonas syringae Strains and PCR Identification in Plants

Alain Bultreys* and Isabelle Gheysen

Departement de Biotechnologie, Centre de Recherches Agronomiques de Gembloux, Ministère des Classes Moyennes et de l'Agriculture, B-5030 Gembloux, Belgium

Received 16 November 1998/Accepted 14 February 1999

Toxin-based identification procedures are useful for differentiating Pseudomonas syringae pathovars. A biological test on peptone-glucose-NaCl agar in which the yeast Rhodotorula pilimanae was used proved to be more reliable for detecting lipodepsipeptide-producing strains of P. syringae than the more usual test on potato dextrose agar in which Geotrichum candidum is used. A PCR test performed with primers designed to amplify a 1,040-bp fragment in the coding sequence of the syrD gene, which was assumed to be involved in syringomycin and syringopeptin secretion, efficiently detected the gene in pathovars that produce the lipodepsipeptides. Comparable results were obtained in both tests performed with strains of the syringomycin-producing organisms P. syringae pv. syringae, P. syringae pv. atrofaciens, and P. syringae pv. aptata, but the PCR test failed with a syringotoxin-producing Pseudomonas fuscovaginae strain. The specificity of the test was verified by obtaining negative PCR test results for related pathovars or species that do not produce the toxic lipodepsipeptides. P. syringae pv. syringae was detected repeatedly in liquid medium inoculated with diseased vegetative tissue and assayed by the PCR test. Our procedure was also adapted to detect P. syringae pv. morsprunorum with a cfl gene-based PCR test.

* Corresponding author. Mailing address: Département de Biotechnologie, Centre de Recherches Agronomiques de Gembloux, 234 Chaussée de Charleroi, B-5030 Gembloux, Belgium. Phone: (32) 81 61 29 35. Fax: (32) 81 61 04 59. E-mail: bultreys{at}cragx.fgov.be.

Applied and Environmental Microbiology, May 1999, p. 1904-1909, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bultreys, A., Gheysen, I., de Hoffmann, E. (2006). Yersiniabactin Production by Pseudomonas syringae and Escherichia coli, and Description of a Second Yersiniabactin Locus Evolutionary Group.. Appl. Environ. Microbiol. 72: 3814-3825 [Abstract] [Full Text]  
  • Hwang, M. S. H., Morgan, R. L., Sarkar, S. F., Wang, P. W., Guttman, D. S. (2005). Phylogenetic Characterization of Virulence and Resistance Phenotypes of Pseudomonas syringae. Appl. Environ. Microbiol. 71: 5182-5191 [Abstract] [Full Text]  
  • Bultreys, A., Gheysen, I., Wathelet, B., Maraite, H., de Hoffmann, E. (2003). High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species. Appl. Environ. Microbiol. 69: 1143-1153 [Abstract] [Full Text]  
  • Bultreys, A., Gheysen, I., Maraite, H., de Hoffmann, E. (2001). Characterization of Fluorescent and Nonfluorescent Peptide Siderophores Produced by Pseudomonas syringae Strains and Their Potential Use in Strain Identification. Appl. Environ. Microbiol. 67: 1718-1727 [Abstract] [Full Text]  
  • Bultreys, A., Gheysen, I. (2000). Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352. Appl. Environ. Microbiol. 66: 325-331 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»