Previous Article | Next Article 
Applied and Environmental Microbiology, May 1999, p. 1936-1940, Vol. 65, No. 5
Departamento de Protección Vegetal y
Biotecnología, Instituto Valenciano de Investigaciones
Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain
Received 30 October 1998/Accepted 12 February 1999
The crown gall biocontrol agent strain K84 and three mutants
derived from it, K1026 (Tra
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Cocolonization of the Rhizosphere by Pathogenic
Agrobacterium Strains and Nonpathogenic Strains K84 and
K1026, Used for Crown Gall Biocontrol
deletion mutant of pAgK84),
K84 Agr (lacking pAgK84), and K1143 (lacking pAgK84 and
pNoc), significantly reduced gall formation caused by two pathogenic
strains resistant to agrocin 84 in peach × almond seedlings
planted in infested soil. Cocolonization of roots by pathogenic and
nonpathogenic strains was observed in these biocontrol experiments
under field conditions. In spite of the efficient biocontrol observed,
average populations consisting of 102 and 106
pathogenic agrobacteria per g of root were found 8 months after planting. The total numbers of pathogenic bacteria on roots were similar for plants treated with the biocontrol strains and for the
untreated plants. Strain K84 and the genetically engineered organism
K1026 survived at a level of 106 agrocin 84-producing
bacteria per g of root. The population size of genetically engineered
strain K1026 was not significantly different than the population size
of wild-type strain K84 8 months after root inoculation. Strains K84
and K1026 controlled two pathogens resistant to agrocin 84 without
reducing the total number of pathogenic bacteria in the root system. In
addition, this study shows that some biological control activity of
strain K84 against agrocin 84-resistant pathogens is independent of
plasmids pAgK84 and pNoc.
*
Corresponding author. Mailing address: Departamento de
Protección Vegetal y Biotecnologia, Instituto Valenciano de
Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia,
Spain. Phone: 34-96-1391000. Fax: 34-96-1390240. E-mail:
mlopez{at}master.ivia.es.
Applied and Environmental Microbiology, May 1999, p. 1936-1940, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Escobar, M. A., Civerolo, E. L., Summerfelt, K. R., Dandekar, A. M.
(2001). RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis. Proc. Natl. Acad. Sci. USA
10.1073/pnas.241276898v1
[Abstract] [Full Text] -
Whipps, J. M.
(2001). Microbial interactions and biocontrol in the rhizosphere. J Exp Bot
52: 487-511
[Abstract] [Full Text] -
Penyalver, R., Oger, P., López, M. M., Farrand, S. K.
(2001). Iron-Binding Compounds from Agrobacterium spp.: Biological Control Strain Agrobacterium rhizogenes K84 Produces a Hydroxamate Siderophore. Appl. Environ. Microbiol.
67: 654-664
[Abstract] [Full Text] -
Escobar, M. A., Civerolo, E. L., Summerfelt, K. R., Dandekar, A. M.
(2001). RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis. Proc. Natl. Acad. Sci. USA
98: 13437-13442
[Abstract] [Full Text]
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»