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Applied and Environmental Microbiology, May 1999, p. 2136-2142, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Growth from Spores of Nonproteolytic Clostridium botulinum in Heat-Treated Vegetable Juice

Sandra C. Stringer,* Nuzrul Haque, and Michael W. Peck

Genetics and Microbiology Department, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, United Kingdom

Received 23 September 1998/Accepted 10 February 1999

Unheated spores of nonproteolytic Clostridium botulinum were able to lead to growth in sterile deoxygenated turnip, spring green, helda bean, broccoli, or potato juice, although the probability of growth was low and the time to growth was longer than the time to growth in culture media. With all five vegetable juices tested, the probability of growth increased when spores were inoculated into the juice and then heated for 2 min in a water bath at 80°C. The probability of growth was greater in bean or broccoli juice than in culture media following 10 min of heat treatment in these media. Growth was prevented by heat treatment of spores in vegetable juices or culture media at 80°C for 100 min. We show for the first time that adding heat-treated vegetable juice to culture media can increase the number of heat-damaged spores of C. botulinum that can lead to colony formation.

* Corresponding author. Mailing address: Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich, NR4 7UA, United Kingdom. Phone: 44 (0) 1603 255000. Fax: 44 (0) 1603 507723. E-mail: Sandra.Stringer{at}BBSRC.AC.UK.

Applied and Environmental Microbiology, May 1999, p. 2136-2142, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Stringer, S. C., Webb, M. D., Peck, M. W. (2009). Contrasting Effects of Heat Treatment and Incubation Temperature on Germination and Outgrowth of Individual Spores of Nonproteolytic Clostridium botulinum Bacteria. Appl. Environ. Microbiol. 75: 2712-2719 [Abstract] [Full Text]  
  • Webb, M. D., Pin, C., Peck, M. W., Stringer, S. C. (2007). Historical and Contemporary NaCl Concentrations Affect the Duration and Distribution of Lag Times from Individual Spores of Nonproteolytic Clostridium botulinum. Appl. Environ. Microbiol. 73: 2118-2127 [Abstract] [Full Text]  
  • Fernández, P. S., Peck, M. W. (1999). A Predictive Model That Describes the Effect of Prolonged Heating at 70 to 90{degrees}C and Subsequent Incubation at Refrigeration Temperatures on Growth from Spores and Toxigenesis by Nonproteolytic Clostridium botulinum in the Presence of Lysozyme. Appl. Environ. Microbiol. 65: 3449-3457 [Abstract] [Full Text]  


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