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Applied and Environmental Microbiology, May 1999, p. 2235-2237, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Salmonella spp. and Listeria monocytogenes in Suspended Organic Waste by Nucleic Acid Extraction and PCR

Carola Burtscher, Papa A. Fall, Peter A. Wilderer, and Stefan Wuertz*

Institute of Water Quality Control and Waste Management, Technical University of Munich, Am Coulombwall, D-85748 Garching, Germany

Received 2 October 1998/Accepted 27 January 1999

A nucleic acid-based method for the detection of the bacterial pathogens Salmonella spp. and Listeria monocytogenes in biological waste was developed. The detection limits were less than 10 cells per ml of biological waste. The method does not include a phenol extraction step and can be easily performed in 1 to 2 days.

* Corresponding author. Mailing address: Institute of Water Quality Control and Waste Management, Technical University of Munich, Am Coulombwall, D-85748 Garching, Germany. Phone: 49 (89) 2891 3708. Fax: 49 (89) 2891 3718. E-mail: stefan.wuertz{at}wga.bauwesen.tu-muenchen.de.

Applied and Environmental Microbiology, May 1999, p. 2235-2237, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Burtscher, C., Wuertz, S. (2003). Evaluation of the Use of PCR and Reverse Transcriptase PCR for Detection of Pathogenic Bacteria in Biosolids from Anaerobic Digestors and Aerobic Composters. Appl. Environ. Microbiol. 69: 4618-4627 [Abstract] [Full Text]  


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