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Applied and Environmental Microbiology, May 1999, p. 2256-2259, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Microbial Biomass and Activity in Lead-Contaminated Soil

A. Konopka,1,* T. Zakharova,1,dagger M. Bischoff,2 L. Oliver,2 C. Nakatsu,2 and R. F. Turco2

Department of Biological Sciences,1 and Department of Agronomy,2 Purdue University, West Lafayette, Indiana 47907

Received 10 August 1998/Accepted 15 February 1999

Microbial community diversity, potential microbial activity, and metal resistance were determined in three soils whose lead contents ranged from 0.00039 to 48 mmol of Pb kg of soil-1. Biomass levels were directly related to lead content. A molecular analysis of 16S rRNAs suggested that each soil contained a complex, diverse microbial community. A statistical analysis of the phospholipid fatty acids indicated that the community in the soil having the highest lead content was not related to the communities in the other soils. All of the soils contained active microbial populations that mineralized [14C]glucose. In all samples, 10 to 15% of the total culturable bacteria were Pb resistant and had MIC of Pb for growth of 100 to 150 µM.

* Corresponding author. Mailing address: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392. Phone: (765) 494-8152. Fax: (765) 494-0876. E-mail: akonopka{at}purdue.edu.

dagger Permanent address: Institute of Soil Science and Photosynthesis, Russian Academy of Science, Pushchino, Russia.

Applied and Environmental Microbiology, May 1999, p. 2256-2259, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nakatsu, C. H., Carmosini, N., Baldwin, B., Beasley, F., Kourtev, P., Konopka, A. (2005). Soil Microbial Community Responses to Additions of Organic Carbon Substrates and Heavy Metals (Pb and Cr). Appl. Environ. Microbiol. 71: 7679-7689 [Abstract] [Full Text]  
  • Feris, K., Ramsey, P., Frazar, C., Moore, J. N., Gannon, J. E., Holben, W. E. (2003). Differences in Hyporheic-Zone Microbial Community Structure along a Heavy-Metal Contamination Gradient. Appl. Environ. Microbiol. 69: 5563-5573 [Abstract] [Full Text]  
  • Nakatsu, C. H., Torsvik, V., Øvreås, L. (2000). Soil Community Analysis Using DGGE of 16S rDNA Polymerase Chain Reaction Products. Soil Sci. 64: 1382-1388 [Abstract] [Full Text]  


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