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Applied and Environmental Microbiology, May 1999, p. 2272-2275, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Stability of Related Human and Chicken Campylobacter jejuni Genotypes after Passage through Chick Intestine Studied by Pulsed-Field Gel Electrophoresis

Marja-Liisa Hänninen,1,* Marjaana Hakkinen,2 and Hilpi Rautelin3,4

Department of Food and Environmental Hygiene1 and Department of Bacteriology and Immunology, The Haartman Institute,3 and Helsinki University Central Hospital Diagnostics,4 FIN-00014 University of Helsinki, and National Veterinary and Food Research Institute, 00231 Helsinki,2 Finland

Received 19 October 1998/Accepted 3 March 1999

The genomic stability of 12 Campylobacter jejuni strains consisting of two groups of human and chicken isolates was studied by analysis of their PFGE (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks' intestines. The patterns of SmaI, SalI, and SacII digests remained stable after intestinal passage, except for those of two strains. One originally human strain, FB 6371, changed its genotype from II/A (SmaI/SacII) to I/B. Another strain, BTI, originally isolated from a chicken, changed its genotype from I/B to a new genotype. The genomic instability of the strains was further confirmed by SalI digestion and ribotyping of the HaeIII digests. In addition, heat-stable serotype 57 of strain FB 6371 changed to serotype 27 in all isolates with new genotypes but remained unchanged in an isolate with the original genotype. Serotype 27 of strain BTI remained stable. Our study suggests that during intestinal colonization, genomic rearrangement, as demonstrated by changed PFGE and ribopatterns, may occur.

* Corresponding author. Mailing address: Faculty of Veterinary Medicine, Department of Food and Environmental Hygiene, P.O. Box 57, FIN-00014 University of Helsinki, Finland. Phone: 358-9-70849704. Fax: 358-9-70849718. E-mail: marja-liisa.hanninen{at}helsinki.fi.

Applied and Environmental Microbiology, May 1999, p. 2272-2275, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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