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Applied and Environmental Microbiology, May 1999, p. 2272-2275, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Stability of Related Human and Chicken
Campylobacter jejuni Genotypes after Passage through Chick
Intestine Studied by Pulsed-Field Gel Electrophoresis
Marja-Liisa
Hänninen,1,*
Marjaana
Hakkinen,2 and
Hilpi
Rautelin3,4
Department of Food and Environmental
Hygiene1 and Department of Bacteriology
and Immunology, The Haartman Institute,3 and
Helsinki University Central Hospital
Diagnostics,4 FIN-00014 University of Helsinki,
and National Veterinary and Food Research Institute, 00231 Helsinki,2 Finland
Received 19 October 1998/Accepted 3 March 1999
The genomic stability of 12 Campylobacter jejuni
strains consisting of two groups of human and chicken isolates was
studied by analysis of their PFGE (pulsed-field gel electrophoresis)
patterns after passage through newly hatched chicks' intestines. The
patterns of SmaI, SalI, and SacII
digests remained stable after intestinal passage, except for those of
two strains. One originally human strain, FB 6371, changed its genotype
from II/A (SmaI/SacII) to I/B. Another strain,
BTI, originally isolated from a chicken, changed its genotype from I/B
to a new genotype. The genomic instability of the strains was further
confirmed by SalI digestion and ribotyping of the
HaeIII digests. In addition, heat-stable serotype 57 of strain FB 6371 changed to serotype 27 in all isolates with new genotypes but remained unchanged in an isolate with the original genotype. Serotype 27 of strain BTI remained stable. Our study suggests
that during intestinal colonization, genomic rearrangement, as
demonstrated by changed PFGE and ribopatterns, may occur.
*
Corresponding author. Mailing address: Faculty of
Veterinary Medicine, Department of Food and Environmental Hygiene, P.O. Box 57, FIN-00014 University of Helsinki, Finland. Phone:
358-9-70849704. Fax: 358-9-70849718. E-mail:
marja-liisa.hanninen{at}helsinki.fi.
Applied and Environmental Microbiology, May 1999, p. 2272-2275, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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