This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Sullivan, E.
Right arrow Articles by Condon, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Sullivan, E.
Right arrow Articles by Condon, S.
Agricola
Right arrow Articles by O'Sullivan, E.
Right arrow Articles by Condon, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 1999, p. 2287-2293, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Relationship between Acid Tolerance, Cytoplasmic pH, and ATP and H+-ATPase Levels in Chemostat Cultures of Lactococcus lactis

Eilís O'Sullivan and Séamus Condon*

Department of Microbiology, University College Cork, Cork, Ireland

Received 5 October 1998/Accepted 26 February 1999

The acid tolerance response (ATR) of chemostat cultures of Lactococcus lactis subsp. cremoris NCDO 712 was dependent on the dilution rate and on the extracellular pH (pHo). A decrease in either the dilution rate or the pHo led to a decrease in the cytoplasmic pH (pHi) of the cells, and similar levels of acid tolerance were observed at any specific pHi irrespective of whether the pHi resulted from manipulation of the growth rate, manipulation of the pHo, or both. Acid tolerance was also induced by sudden additions of acid to chemostat cultures growing at a pHo of 7.0, and this induction was completely inhibited by chloramphenicol. The end products of glucose fermentation depended on the growth rate and the environmental pHo of the cultures, but neither the spectrum of end products nor the total rate of acid production correlated with a specific pHi. The rate of ATP formation was not correlated with pHi, but a good correlation between the cellular level of H+-ATPase and pHi was observed. Moreover, an inverse correlation between the cytoplasmic levels of ATP and pHi was established. Each pHi below 6.6 was characterized by unique levels of ATR, H+-ATPase, and ATP. High levels of H+-ATPase also coincided with high levels of acid tolerance of cells in batch cultures induced with sublethal levels of acid. We concluded that H+-ATPase is one of the ATR proteins induced by acid pHi through growth at an acid pHo or a slow growth rate.

* Corresponding author. Mailing address: Department of Microbiology, University College Cork, Cork, Ireland. Phone: 353 21 902396. Fax: 353 21 903101. E-mail: s.condon{at}ucc.ie.

Applied and Environmental Microbiology, June 1999, p. 2287-2293, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sanchez, C., Neves, A. R., Cavalheiro, J., dos Santos, M. M., Garcia-Quintans, N., Lopez, P., Santos, H. (2008). Contribution of Citrate Metabolism to the Growth of Lactococcus lactis CRL264 at Low pH. Appl. Environ. Microbiol. 74: 1136-1144 [Abstract] [Full Text]  
  • Sanchez, B., Champomier-Verges, M.-C., Collado, M. d. C., Anglade, P., Baraige, F., Sanz, Y., de los Reyes-Gavilan, C. G., Margolles, A., Zagorec, M. (2007). Low-pH Adaptation and the Acid Tolerance Response of Bifidobacterium longum Biotype longum. Appl. Environ. Microbiol. 73: 6450-6459 [Abstract] [Full Text]  
  • Zhang, J., Fu, R.-Y., Hugenholtz, J., Li, Y., Chen, J. (2007). Glutathione Protects Lactococcus lactis against Acid Stress. Appl. Environ. Microbiol. 73: 5268-5275 [Abstract] [Full Text]  
  • Papadimitriou, K., Pratsinis, H., Nebe-von-Caron, G., Kletsas, D., Tsakalidou, E. (2007). Acid Tolerance of Streptococcus macedonicus as Assessed by Flow Cytometry and Single-Cell Sorting. Appl. Environ. Microbiol. 73: 465-476 [Abstract] [Full Text]  
  • Penaud, S., Fernandez, A., Boudebbouze, S., Ehrlich, S. D., Maguin, E., van de Guchte, M. (2006). Induction of Heavy-Metal-Transporting CPX-Type ATPases during Acid Adaptation in Lactobacillus bulgaricus. Appl. Environ. Microbiol. 72: 7445-7454 [Abstract] [Full Text]  
  • Larsen, N., Boye, M., Siegumfeldt, H., Jakobsen, M. (2006). Differential Expression of Proteins and Genes in the Lag Phase of Lactococcus lactis subsp. lactis Grown in Synthetic Medium and Reconstituted Skim Milk. Appl. Environ. Microbiol. 72: 1173-1179 [Abstract] [Full Text]  
  • Fu, R.-Y., Chen, J., Li, Y. (2005). Heterologous Leaky Production of Transglutaminase in Lactococcus lactis Significantly Enhances the Growth Performance of the Host. Appl. Environ. Microbiol. 71: 8911-8919 [Abstract] [Full Text]  
  • McEntire, J. C., Carman, G. M., Montville, T. J. (2004). Increased ATPase Activity Is Responsible for Acid Sensitivity of Nisin-Resistant Listeria monocytogenes ATCC 700302. Appl. Environ. Microbiol. 70: 2717-2721 [Abstract] [Full Text]  
  • Even, S., Lindley, N. D., Cocaign-Bousquet, M. (2003). Transcriptional, translational and metabolic regulation of glycolysis in Lactococcus lactis subsp. cremoris MG 1363 grown in continuous acidic cultures. Microbiology 149: 1935-1944 [Abstract] [Full Text]  
  • Shabala, L., Budde, B., Ross, T., Siegumfeldt, H., Jakobsen, M., McMeekin, T. (2002). Responses of Listeria monocytogenes to Acid Stress and Glucose Availability Revealed by a Novel Combination of Fluorescence Microscopy and Microelectrode Ion-Selective Techniques. Appl. Environ. Microbiol. 68: 1794-1802 [Abstract] [Full Text]  
  • De Angelis, M., Bini, L., Pallini, V., Cocconcelli, P. S., Gobbetti, M. (2001). The acid-stress response in Lactobacillus sanfranciscensis CB1. Microbiology 147: 1863-1873 [Abstract] [Full Text]  
  • Desvaux, M., Guedon, E., Petitdemange, H. (2001). Metabolic flux in cellulose batch and cellulose-fed continuous cultures of Clostridium cellulolyticum in response to acidic environment. Microbiology 147: 1461-1471 [Abstract] [Full Text]  
  • Siegumfeldt, H., Björn Rechinger, K., Jakobsen, M. (2000). Dynamic Changes of Intracellular pH in Individual Lactic Acid Bacterium Cells in Response to a Rapid Drop in Extracellular pH. Appl. Environ. Microbiol. 66: 2330-2335 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»