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Applied and Environmental Microbiology, June 1999, p. 2363-2368, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transcriptional Control of ADH Genes in the Xylose-Fermenting Yeast Pichia stipitis

Jae-Yong Chodagger and Thomas W. Jeffries*

Forest Products Laboratory, U.S. Department of Agriculture, Forest Service, Madison, Wisconsin 53705, and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

Received 15 December 1998/Accepted 5 April 1999

We studied the expression of the genes encoding group I alcohol dehydrogenases (PsADH1 and PsADH2) in the xylose-fermenting yeast Pichia stipitis CBS 6054. The cells expressed PsADH1 approximately 10 times higher under oxygen-limited conditions than under fully aerobic conditions when cultivated on xylose. Transcripts of PsADH2 were not detectable under either aeration condition. We used a PsADH1::lacZ fusion to monitor PsADH1 expression and found that expression increased as oxygen decreased. The level of PsADH1 transcript was repressed about 10-fold in cells grown in the presence of heme under oxygen-limited conditions. Concomitantly with the induction of PsADH1, PsCYC1 expression was repressed. These results indicate that oxygen availability regulates PsADH1 expression and that regulation may be mediated by heme. The regulation of PsADH2 expression was also examined in other genetic backgrounds. Disruption of PsADH1 dramatically increased PsADH2 expression on nonfermentable carbon sources under fully aerobic conditions, indicating that the expression of PsADH2 is subject to feedback regulation under these conditions.

* Corresponding author. Mailing address: Institute for Microbial and Biochemical Technology, Forest Products Laboratory, One Gifford Pinchot Dr., Madison, WI 53705. Phone: (608) 231-9453. Fax: (608) 231-9262. E-mail: twjeffri{at}facstaff.wisc.edu.

dagger Present address: Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Frederick, MD 21702-1201.

Applied and Environmental Microbiology, June 1999, p. 2363-2368, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lu, C., Jeffries, T. (2007). Shuffling of Promoters for Multiple Genes To Optimize Xylose Fermentation in an Engineered Saccharomyces cerevisiae Strain. Appl. Environ. Microbiol. 73: 6072-6077 [Abstract] [Full Text]  
  • Ilmen, M., Koivuranta, K., Ruohonen, L., Suominen, P., Penttila, M. (2007). Efficient Production of L-Lactic Acid from Xylose by Pichia stipitis. Appl. Environ. Microbiol. 73: 117-123 [Abstract] [Full Text]  
  • Jin, Y.-S., Laplaza, J. M., Jeffries, T. W. (2004). Saccharomyces cerevisiae Engineered for Xylose Metabolism Exhibits a Respiratory Response. Appl. Environ. Microbiol. 70: 6816-6825 [Abstract] [Full Text]  
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