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Applied and Environmental Microbiology, June 1999, p. 2446-2452, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Pyruvated Mannose-Specific Xanthan Lyase Involved in Xanthan Degradation by Paenibacillus alginolyticus XL-1

Harald J. Ruijssenaars,* Jan A. M. de Bont, and Sybe Hartmans

Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, 6700 EV Wageningen, The Netherlands

Received 14 December 1998/Accepted 24 March 1999

The xanthan-degrading bacterium Paenibacillus alginolyticus XL-1, isolated from soil, degrades approximately 28% of the xanthan molecule and appears to leave the backbone intact. Several xanthan-degrading enzymes were excreted during growth on xanthan, including xanthan lyase. Xanthan lyase production was induced by xanthan and inhibited by glucose and low-molecular-weight enzymatic degradation products from xanthan. A xanthan lyase with a molecular mass of 85 kDa and a pI of 7.9 was purified and characterized. The enzyme is specific for pyruvated mannosyl side chain residues and optimally active at pH 6.0 and 55°C.

* Corresponding author. Mailing address: Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Phone: 31-317484980. Fax: 31-317484978. E-mail: Harald.Ruijssenaars{at}imb.ftns.wau.nl.

Applied and Environmental Microbiology, June 1999, p. 2446-2452, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Ruijssenaars, H. J., Hartmans, S., Verdoes, J. C. (2000). A Novel Gene Encoding Xanthan Lyase of Paenibacillus alginolyticus Strain XL-1. Appl. Environ. Microbiol. 66: 3945-3950 [Abstract] [Full Text]  
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