This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Vries, R. P.
Right arrow Articles by Visser, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Vries, R. P.
Right arrow Articles by Visser, J.
Agricola
Right arrow Articles by de Vries, R. P.
Right arrow Articles by Visser, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 1999, p. 2453-2460, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differential Expression of Three alpha -Galactosidase Genes and a Single beta -Galactosidase Gene from Aspergillus niger

Ronald P. de Vries, Hetty C. van den Broeck, Ester Dekkers, Paloma Manzanares,dagger Leo H. de Graaff, and Jaap Visser*

Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, NL-6703 HA Wageningen, The Netherlands

Received 4 January 1999/Accepted 1 April 1999

A gene encoding a third alpha -galactosidase (AglB) from Aspergillus niger has been cloned and sequenced. The gene consists of an open reading frame of 1,750 bp containing six introns. The gene encodes a protein of 443 amino acids which contains a eukaryotic signal sequence of 16 amino acids and seven putative N-glycosylation sites. The mature protein has a calculated molecular mass of 48,835 Da and a predicted pI of 4.6. An alignment of the AglB amino acid sequence with those of other alpha -galactosidases revealed that it belongs to a subfamily of alpha -galactosidases that also includes A. niger AglA. A. niger AglC belongs to a different subfamily that consists mainly of prokaryotic alpha -galactosidases. The expression of aglA, aglB, aglC, and lacA, the latter of which encodes an A. niger beta -galactosidase, has been studied by using a number of monomeric, oligomeric, and polymeric compounds as growth substrates. Expression of aglA is only detected on galactose and galactose-containing oligomers and polymers. The aglB gene is expressed on all of the carbon sources tested, including glucose. Elevated expression was observed on xylan, which could be assigned to regulation via XlnR, the xylanolytic transcriptional activator. Expression of aglC was only observed on glucose, fructose, and combinations of glucose with xylose and galactose. High expression of lacA was detected on arabinose, xylose, xylan, and pectin. Similar to aglB, the expression on xylose and xylan can be assigned to regulation via XlnR. All four genes have distinct expression patterns which seem to mirror the natural substrates of the encoded proteins.

* Corresponding author. Mailing address: Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands. Phone: 31 (0) 317 482865. Fax: 31 (0) 317 484011. E-mail: office{at}algemeen.mgim.wau.nl.

dagger Present address: Instituto de Agroquimica y Technologia de Alimentos CSIC, 46100 Burjassot, Valencia, Spain.

Applied and Environmental Microbiology, June 1999, p. 2453-2460, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fekete, E., Karaffa, L., Kubicek, C. P., Szentirmai, A., Seiboth, B. (2007). Induction of extracellular beta-galactosidase (Bga1) formation by D-galactose in Hypocrea jecorina is mediated by galactitol. Microbiology 153: 507-512 [Abstract] [Full Text]  
  • Seiboth, B., Hartl, L., Salovuori, N., Lanthaler, K., Robson, G. D., Vehmaanpera, J., Penttila, M. E., Kubicek, C. P. (2005). Role of the bga1-Encoded Extracellular {beta}-Galactosidase of Hypocrea jecorina in Cellulase Induction by Lactose. Appl. Environ. Microbiol. 71: 851-857 [Abstract] [Full Text]  
  • de Vries, R. P., Visser, J. (2001). Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides. Microbiol. Mol. Biol. Rev. 65: 497-522 [Abstract] [Full Text]  
  • Feurtado, J. A., Banik, M., Bewley, J. D. (2001). The cloning and characterization of {{alpha}}-galactosidase present during and following germination of tomato (Lycopersicon esculentum Mill.) seed. J Exp Bot 52: 1239-1249 [Abstract] [Full Text]  
  • Fridjonsson, O., Watzlawick, H., Gehweiler, A., Rohrhirsch, T., Mattes, R. (1999). Cloning of the Gene Encoding a Novel Thermostable alpha -Galactosidase from Thermus brockianus ITI360. Appl. Environ. Microbiol. 65: 3955-3963 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»