This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hage, J. C.
Right arrow Articles by Hartmans, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hage, J. C.
Right arrow Articles by Hartmans, S.
Agricola
Right arrow Articles by Hage, J. C.
Right arrow Articles by Hartmans, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 1999, p. 2466-2470, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Monooxygenase-Mediated 1,2-Dichloroethane Degradation by Pseudomonas sp. Strain DCA1

Jacobus C. Hage* and Sybe Hartmans

Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, 6700 EV Wageningen, The Netherlands

Received 30 November 1998/Accepted 22 March 1999

A bacterial strain, designated Pseudomonas sp. strain DCA1, was isolated from a 1,2-dichloroethane (DCA)-degrading biofilm. Strain DCA1 utilizes DCA as the sole carbon and energy source and does not require additional organic nutrients, such as vitamins, for optimal growth. The affinity of strain DCA1 for DCA is very high, with a Km value below the detection limit of 0.5 µM. Instead of a hydrolytic dehalogenation, as in other DCA utilizers, the first step in DCA degradation in strain DCA1 is an oxidation reaction. Oxygen and NAD(P)H are required for this initial step. Propene was converted to 1,2-epoxypropane by DCA-grown cells and competitively inhibited DCA degradation. We concluded that a monooxygenase is responsible for the first step in DCA degradation in strain DCA1. Oxidation of DCA probably results in the formation of the unstable intermediate 1,2-dichloroethanol, which spontaneously releases chloride, yielding chloroacetaldehyde. The DCA degradation pathway in strain DCA1 proceeds from chloroacetaldehyde via chloroacetic acid and presumably glycolic acid, which is similar to degradation routes observed in other DCA-utilizing bacteria.

* Corresponding author. Mailing address: Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Phone: 31-317-483393. Fax: 31-317-484978. E-mail: Ko.Hage{at}imb.ftns.wau.nl.

Applied and Environmental Microbiology, June 1999, p. 2466-2470, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jennings, L. K., Chartrand, M. M. G., Lacrampe-Couloume, G., Lollar, B. S., Spain, J. C., Gossett, J. M. (2009). Proteomic and Transcriptomic Analyses Reveal Genes Upregulated by cis-Dichloroethene in Polaromonas sp. Strain JS666. Appl. Environ. Microbiol. 75: 3733-3744 [Abstract] [Full Text]  
  • Mena-Benitez, G. L., Gandia-Herrero, F., Graham, S., Larson, T. R., McQueen-Mason, S. J., French, C. E., Rylott, E. L., Bruce, N. C. (2008). Engineering a Catabolic Pathway in Plants for the Degradation of 1,2-Dichloroethane. Plant Physiol. 147: 1192-1198 [Abstract] [Full Text]  
  • Hunkeler, D., Aravena, R. (2000). Evidence of Substantial Carbon Isotope Fractionation among Substrate, Inorganic Carbon, and Biomass during Aerobic Mineralization of 1,2-Dichloroethane by Xanthobacter autotrophicus. Appl. Environ. Microbiol. 66: 4870-4876 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»