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Applied and Environmental Microbiology, June 1999, p. 2492-2496, Vol. 65, No. 6
0099-2240/99/$04.00+0

Occurrence of Nontuberculous Mycobacteria in Environmental Samples

Terry C. Covert,* Mark R. Rodgers, Antolin L. Reyes, and Gerard N. Stelma Jr.

National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268

Received 2 November 1998/Accepted 16 March 1999

Nontuberculous mycobacteria (NTM) are a major cause of opportunistic infection in immunocompromised hosts. Because there is no evidence of person-to-person transmission and NTM have been found in drinking water, the environment is considered a likely source of infection. In this study the widespread occurrence of NTM was examined in drinking water, bottled water, and ice samples. A total of 139 samples were examined for NTM by a membrane filtration culture technique followed by PCR amplification and 16S rRNA sequence determination to identify the isolates. NTM were not detected in bottled water or cisterns but were detected in 54% of the ice samples and 35% of the public drinking-water samples from 21 states. The most frequently occurring isolate was M. mucogenicum (formerly referred to as an M. chelonae-like organism).

* Corresponding author. Mailing address: U.S. Environmental Protection Agency, National Exposure Research Laboratory, 26 W. Martin Luther King Dr., Cincinnati, OH 45268. Phone: (513) 569-7318. Fax: (513) 569-7117. E-mail: Covert.Terry{at}epamail.epa.gov.

Applied and Environmental Microbiology, June 1999, p. 2492-2496, Vol. 65, No. 6
0099-2240/99/$04.00+0


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