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Applied and Environmental Microbiology, June 1999, p. 2598-2605, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mannan-Degrading Enzymes from Cellulomonas fimi

Dominik Stoll, Henrik Stålbrand,dagger and R. Antony J. Warren*

Department of Microbiology and Immunology and The Protein Engineering Network of Centres of Excellence, The University of British Columbia, Vancouver, British Columbia, Canada

Received 15 December 1998/Accepted 22 February 1999

The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and beta -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian beta -mannosidases.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Blvd., Vancouver, BC, Canada V6T 1Z3. Phone: (604) 822-2376. Fax: (604) 822-6041. E-mail: rajw{at}unixg.ubc.ca.

dagger Present address: Department of Biochemistry, University of Lund, Lund, Sweden.

Applied and Environmental Microbiology, June 1999, p. 2598-2605, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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