Previous Article | Next Article ![]()
Applied and Environmental Microbiology, June 1999, p. 2598-2605, Vol. 65, No. 6
Department of Microbiology and Immunology and
The Protein Engineering Network of Centres of Excellence, The
University of British Columbia, Vancouver, British Columbia, Canada
Received 15 December 1998/Accepted 22 February 1999
The genes man26a and man2A from
Cellulomonas fimi encode mannanase 26A (Man26A) and
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Mannan-Degrading Enzymes from
Cellulomonas fimi
and
-mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted,
modular protein of 951 amino acids, comprising a catalytic module in
family 26 of glycosyl hydrolases, an S-layer homology module, and two
modules of unknown function. Exposure of Man26A produced by
Escherichia coli to C. fimi protease generates
active fragments of the enzyme that correspond to polypeptides with
mannanase activity produced by C. fimi during growth on
mannans, indicating that it may be the only mannanase produced by the
organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme
comprising a catalytic module in a subfamily of family 2 of the
glycosyl hydrolases that at present contains only mammalian
-mannosidases.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, University of British Columbia, 300-6174 University Blvd., Vancouver, BC, Canada V6T 1Z3. Phone: (604) 822-2376. Fax: (604) 822-6041. E-mail: rajw{at}unixg.ubc.ca.
Present address: Department of Biochemistry, University of Lund,
Lund, Sweden.
This article has been cited by other articles:
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»