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Applied and Environmental Microbiology, June 1999, p. 2636-2643, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of the Binding Protein-Dependent Cellobiose and Cellotriose Transport System of the Cellulose Degrader Streptomyces reticuli

Andreas Schlösser,* Jens Jantos, Karl Hackmann, and Hildgund Schrempf

FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany

Received 30 November 1998/Accepted 3 March 1999

Streptomyces reticuli has an inducible ATP-dependent uptake system specific for cellobiose and cellotriose. By reversed genetics a gene cluster encoding components of a binding protein-dependent cellobiose and cellotriose ABC transporter was cloned and sequenced. The deduced gene products comprise a regulatory protein (CebR), a cellobiose binding lipoprotein (CebE), two integral membrane proteins (CebF and CebG), and the NH2-terminal part of an intracellular beta -glucosidase (BglC). The gene for the ATP binding protein MsiK is not linked to the ceb operon. We have shown earlier that MsiK is part of two different ABC transport systems, one for maltose and one for cellobiose and cellotriose, in S. reticuli and Streptomyces lividans. Transcription of polycistronic cebEFG and bglC mRNAs is induced by cellobiose, whereas the cebR gene is transcribed independently. Immunological experiments showed that CebE is synthesized during growth with cellobiose and that MsiK is produced in the presence of several sugars at high or moderate levels. The described ABC transporter is the first one of its kind and is the only specific cellobiose/cellotriose uptake system of S. reticuli, since insertional inactivation of the cebE gene prevents high-affinity uptake of cellobiose.

* Corresponding author. Mailing address: FB Biologie/Chemie, Universität Osnabrück, Barbarastraße 11, D-49069 Osnabrück, Germany. Phone: 49 541 969 2843. Fax: 49 541 969 2804. E-mail: schloesser{at}biologie.uni-osnabrueck.de.

Applied and Environmental Microbiology, June 1999, p. 2636-2643, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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