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Applied and Environmental Microbiology, June 1999, p. 2738-2740, Vol. 65, No. 6
0099-2240/99/$04.00+0

Interactions of Saprophytic Yeasts with a nor Mutant of Aspergillus flavus

Sui-Sheng T. Hua,* James L. Baker, and Melanie Flores-Espiritu

Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Albany, California 94710

Received 29 June 1998/Accepted 17 March 1999

The nor mutant of Aspergillus flavus has a defective norsolorinic acid reductase, and thus the aflatoxin biosynthetic pathway is blocked, resulting in the accumulation of norsolorinic acid, a bright red-orange pigment. We developed a visual agar plate assay to monitor yeast strains for their ability to inhibit aflatoxin production by visually scoring the accumulation of this pigment of the nor mutant. We identified yeast strains that reduced the red-orange pigment accumulation in the nor mutant. These yeasts also reduced aflatoxin accumulation by a toxigenic strain of A. flavus. These yeasts may be useful for reducing aflatoxin contamination of food commodities.

* Corresponding author. Mailing address: Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St., Albany, CA 94710. Phone: (510) 559-5905. Fax: (510) 559-5777. E-mail: ssth{at}pw.usda.gov.

Applied and Environmental Microbiology, June 1999, p. 2738-2740, Vol. 65, No. 6
0099-2240/99/$04.00+0


This article has been cited by other articles:

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