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Applied and Environmental Microbiology, June 1999, p. 2773-2775, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Virus Passage through Track-Etch Membranes Modified by Salinity and a Nonionic Surfactant

C. David Lytle,1,* Licia B. Routson,1 Nisha B. Jain,2 Matthew R. Myers,1 and Barbara L. Green3

Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 208571; Thomas Jefferson High School for Science and Technology, Alexandria, Virginia 223122; and Corning, Incorporated, Acton, Massachusetts 017203

Received 14 December 1998/Accepted 4 March 1999

Why do viruses sometimes not pass through larger pores in track-etch filters? Increasing the salinity (0.8 to 160 mM Na+) decreased phi X174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyester membranes. Undiminished passage when 0.1% Tween 80 was added implied that nonionic virus adsorption occurred and indicated that high levels of salinity decreased virus passage by decreasing electrostatic repulsion that prevented adsorption.

* Corresponding author. Mailing address: HFZ-112, Center for Devices and Radiological Health, Food and Drug Administration, 12709 Twinbrook Parkway, Rockville, MD 20857. Phone: (301) 443-7184. Fax: (301) 594-6775. E-mail: cdl{at}cdrh.fda.gov.

Applied and Environmental Microbiology, June 1999, p. 2773-2775, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lukasik, J., Scott, T. M., Andryshak, D., Farrah, S. R. (2000). Influence of Salts on Virus Adsorption to Microporous Filters. Appl. Environ. Microbiol. 66: 2914-2920 [Abstract] [Full Text]  


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