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Applied and Environmental Microbiology, June 1999, p. 2794-2797, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A New Biocatalyst for Production of Optically Pure Aryl Epoxides by Styrene Monooxygenase from Pseudomonas fluorescens ST

Patrizia Di Gennaro,1 Andrea Colmegna,1 Enrica Galli,1 Guido Sello,2 Francesca Pelizzoni,2 and Giuseppina Bestetti1,*

Department of Genetics and Biology of Microorganisms1 and Department of Organic and Industrial Chemistry,2 University of Milan, 20133 Milan, Italy

Received 28 December 1998/Accepted 26 March 1999

We developed a biocatalyst by cloning the styrene monooxygenase genes (styA and styB) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corresponding optically pure epoxides. The results of bioconversions indicate the broad substrate preference of styrene monooxygenase and its potential for the production of several fine chemicals.

* Corresponding author. Mailing address: Department of Genetics and Biology of Microorganisms, University of Milan, Via Celoria 26, 20133 Milan, Italy. Phone: 39-2-26605227. Fax: 39-2-2664551. E-mail: Bestetti{at}imiucca.csi.unimi.it.

Applied and Environmental Microbiology, June 1999, p. 2794-2797, Vol. 65, No. 6
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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