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Applied and Environmental Microbiology, July 1999, p. 3142-3147, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Discriminant Analysis of Ribotype Profiles of Escherichia coli for Differentiating Human and Nonhuman Sources of Fecal Pollutiondagger

Salina Parveen,1,2,* Kenneth M. Portier,3 Kevin Robinson,3 Lee Edmiston,4 and Mark L. Tamplin1,2

Department of Family, Youth, and Community Sciences,1 Department of Food Science and Human Nutrition,2 and Department of Statistics,3 University of Florida, Gainesville, Florida 32611-0287, and Department of Environmental Protection, East Point, Florida 323284

Received 19 February 1999/Accepted 26 April 1999

Estuarine waters receive fecal pollution from a variety of sources, including humans and wildlife. Escherichia coli is a ubiquitous bacterium in the intestines of warm-blooded animals and is used as an indicator of fecal pollution. However, its presence does not specifically differentiate sources of pollution. A total of 238 E. coli isolates from human sources (HS) and nonhuman sources (NHS) were collected from the Apalachicola National Estuarine Research Reserve, from associated sewage treatment plants, and directly from animals and tested for ribotype (RT) profile. HS and NHS isolates showed 41 and 61 RT profiles, respectively. At a similarity index of ca. 50%, HS and NHS isolates demonstrated four clusters, with the majority of HS and NHS isolates located in clusters C and D; isolates obtained directly from human and animal feces also could be grouped within these clusters. Discriminant analysis (DA) of RT profiles showed that 97% of the NHS isolates and 100% of the animal fecal isolates were correctly classified. The average rate of correct classification for HS and NHS isolates was 82%. We conclude that DA of RT profiles may be a useful method for identifying HS and NHS fecal pollution and may potentially facilitate management practices.

* Corresponding author. Mailing address: P.O. Box 110287, University of Florida, Gainesville, FL 32611-0287. Phone: (352) 392-1885. Fax: (352) 846-1102. E-mail: mlt{at}gnv.ifas.ufl.edu.

dagger Florida Agricultural Experiment Station journal series no. R-06879.

Applied and Environmental Microbiology, July 1999, p. 3142-3147, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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