This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parveen, S.
Right arrow Articles by Tamplin, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parveen, S.
Right arrow Articles by Tamplin, M. L.
Agricola
Right arrow Articles by Parveen, S.
Right arrow Articles by Tamplin, M. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 1999, p. 3142-3147, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Discriminant Analysis of Ribotype Profiles of Escherichia coli for Differentiating Human and Nonhuman Sources of Fecal Pollutiondagger

Salina Parveen,1,2,* Kenneth M. Portier,3 Kevin Robinson,3 Lee Edmiston,4 and Mark L. Tamplin1,2

Department of Family, Youth, and Community Sciences,1 Department of Food Science and Human Nutrition,2 and Department of Statistics,3 University of Florida, Gainesville, Florida 32611-0287, and Department of Environmental Protection, East Point, Florida 323284

Received 19 February 1999/Accepted 26 April 1999

Estuarine waters receive fecal pollution from a variety of sources, including humans and wildlife. Escherichia coli is a ubiquitous bacterium in the intestines of warm-blooded animals and is used as an indicator of fecal pollution. However, its presence does not specifically differentiate sources of pollution. A total of 238 E. coli isolates from human sources (HS) and nonhuman sources (NHS) were collected from the Apalachicola National Estuarine Research Reserve, from associated sewage treatment plants, and directly from animals and tested for ribotype (RT) profile. HS and NHS isolates showed 41 and 61 RT profiles, respectively. At a similarity index of ca. 50%, HS and NHS isolates demonstrated four clusters, with the majority of HS and NHS isolates located in clusters C and D; isolates obtained directly from human and animal feces also could be grouped within these clusters. Discriminant analysis (DA) of RT profiles showed that 97% of the NHS isolates and 100% of the animal fecal isolates were correctly classified. The average rate of correct classification for HS and NHS isolates was 82%. We conclude that DA of RT profiles may be a useful method for identifying HS and NHS fecal pollution and may potentially facilitate management practices.

* Corresponding author. Mailing address: P.O. Box 110287, University of Florida, Gainesville, FL 32611-0287. Phone: (352) 392-1885. Fax: (352) 846-1102. E-mail: mlt{at}gnv.ifas.ufl.edu.

dagger Florida Agricultural Experiment Station journal series no. R-06879.

Applied and Environmental Microbiology, July 1999, p. 3142-3147, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, J. E., Lim, M. Y., Kim, S. Y., Lee, S., Lee, H., Oh, H.-M., Hur, H.-G., Ko, G. (2009). Molecular Characterization of Bacteriophages for Microbial Source Tracking in Korea. Appl. Environ. Microbiol. 75: 7107-7114 [Abstract] [Full Text]  
  • Dickerson, J. W. Jr., Crozier, J. B., Hagedorn, C., Hassall, A. (2007). Assessment of the 16S-23S rDNA Intergenic Spacer Region in Enterococcus spp. for Microbial Source Tracking. J. Environ. Qual. 36: 1661-1669 [Abstract] [Full Text]  
  • Gourmelon, M., Caprais, M. P., Segura, R., Le Mennec, C., Lozach, S., Piriou, J. Y., Rince, A. (2007). Evaluation of Two Library-Independent Microbial Source Tracking Methods To Identify Sources of Fecal Contamination in French Estuaries. Appl. Environ. Microbiol. 73: 4857-4866 [Abstract] [Full Text]  
  • Kaneene, J. B., Miller, R., Sayah, R., Johnson, Y. J., Gilliland, D., Gardiner, J. C. (2007). Considerations When Using Discriminant Function Analysis of Antimicrobial Resistance Profiles To Identify Sources of Fecal Contamination of Surface Water in Michigan. Appl. Environ. Microbiol. 73: 2878-2890 [Abstract] [Full Text]  
  • Stoeckel, D. M., Harwood, V. J. (2007). Performance, Design, and Analysis in Microbial Source Tracking Studies. Appl. Environ. Microbiol. 73: 2405-2415 [Full Text]  
  • Kirs, M., Smith, D. C. (2007). Multiplex Quantitative Real-Time Reverse Transcriptase PCR for F+-Specific RNA Coliphages: a Method for Use in Microbial Source Tracking. Appl. Environ. Microbiol. 73: 808-814 [Abstract] [Full Text]  
  • Yan, T., Hamilton, M. J., Sadowsky, M. J. (2007). High-Throughput and Quantitative Procedure for Determining Sources of Escherichia coli in Waterways by Using Host-Specific DNA Marker Genes. Appl. Environ. Microbiol. 73: 890-896 [Abstract] [Full Text]  
  • Anderson, M. A., Whitlock, J. E., Harwood, V. J. (2006). Diversity and Distribution of Escherichia coli Genotypes and Antibiotic Resistance Phenotypes in Feces of Humans, Cattle, and Horses. Appl. Environ. Microbiol. 72: 6914-6922 [Abstract] [Full Text]  
  • Blanch, A. R., Belanche-Munoz, L., Bonjoch, X., Ebdon, J., Gantzer, C., Lucena, F., Ottoson, J., Kourtis, C., Iversen, A., Kuhn, I., Moce, L., Muniesa, M., Schwartzbrod, J., Skraber, S., Papageorgiou, G. T., Taylor, H., Wallis, J., Jofre, J. (2006). Integrated Analysis of Established and Novel Microbial and Chemical Methods for Microbial Source Tracking. Appl. Environ. Microbiol. 72: 5915-5926 [Abstract] [Full Text]  
  • Ahmed, W., Neller, R., Katouli, M. (2005). Host Species-Specific Metabolic Fingerprint Database for Enterococci and Escherichia coli and Its Application To Identify Sources of Fecal Contamination in Surface Waters. Appl. Environ. Microbiol. 71: 4461-4468 [Abstract] [Full Text]  
  • Carson, C. A., Christiansen, J. M., Yampara-Iquise, H., Benson, V. W., Baffaut, C., Davis, J. V., Broz, R. R., Kurtz, W. B., Rogers, W. M., Fales, W. H. (2005). Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces. Appl. Environ. Microbiol. 71: 4945-4949 [Abstract] [Full Text]  
  • Anderson, K. L., Whitlock, J. E., Harwood, V. J. (2005). Persistence and Differential Survival of Fecal Indicator Bacteria in Subtropical Waters and Sediments. Appl. Environ. Microbiol. 71: 3041-3048 [Abstract] [Full Text]  
  • Fong, T.-T., Lipp, E. K. (2005). Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools. Microbiol. Mol. Biol. Rev. 69: 357-371 [Abstract] [Full Text]  
  • Sayah, R. S., Kaneene, J. B., Johnson, Y., Miller, R. (2005). Patterns of Antimicrobial Resistance Observed in Escherichia coli Isolates Obtained from Domestic- and Wild-Animal Fecal Samples, Human Septage, and Surface Water. Appl. Environ. Microbiol. 71: 1394-1404 [Abstract] [Full Text]  
  • Hassan, W. M., Wang, S. Y., Ellender, R. D. (2005). Methods To Increase Fidelity of Repetitive Extragenic Palindromic PCR Fingerprint-Based Bacterial Source Tracking Efforts. Appl. Environ. Microbiol. 71: 512-518 [Abstract] [Full Text]  
  • McLellan, S. L. (2004). Genetic Diversity of Escherichia coli Isolated from Urban Rivers and Beach Water. Appl. Environ. Microbiol. 70: 4658-4665 [Abstract] [Full Text]  
  • Ram, J. L., Ritchie, R. P., Fang, J., Gonzales, F. S., Selegean, J. P. (2004). Sequence-Based Source Tracking of Escherichia coli Based on Genetic Diversity of {beta}-Glucuronidase. J. Environ. Qual. 33: 1024-1032 [Abstract] [Full Text]  
  • Yang, H.-H., Vinopal, R. T., Grasso, D., Smets, B. F. (2004). High Diversity among Environmental Escherichia coli Isolates from a Bovine Feedlot. Appl. Environ. Microbiol. 70: 1528-1536 [Abstract] [Full Text]  
  • Seurinck, S., Verstraete, W., Siciliano, S. D. (2003). Use of 16S-23S rRNA Intergenic Spacer Region PCR and Repetitive Extragenic Palindromic PCR Analyses of Escherichia coli Isolates To Identify Nonpoint Fecal Sources. Appl. Environ. Microbiol. 69: 4942-4950 [Abstract] [Full Text]  
  • Wiggins, B. A., Cash, P. W., Creamer, W. S., Dart, S. E., Garcia, P. P., Gerecke, T. M., Han, J., Henry, B. L., Hoover, K. B., Johnson, E. L., Jones, K. C., McCarthy, J. G., McDonough, J. A., Mercer, S. A., Noto, M. J., Park, H., Phillips, M. S., Purner, S. M., Smith, B. M., Stevens, E. N., Varner, A. K. (2003). Use of Antibiotic Resistance Analysis for Representativeness Testing of Multiwatershed Libraries. Appl. Environ. Microbiol. 69: 3399-3405 [Abstract] [Full Text]  
  • McLellan, S. L., Daniels, A. D., Salmore, A. K. (2003). Genetic Characterization of Escherichia coli Populations from Host Sources of Fecal Pollution by Using DNA Fingerprinting. Appl. Environ. Microbiol. 69: 2587-2594 [Abstract] [Full Text]  
  • Carson, C. A., Shear, B. L., Ellersieck, M. R., Schnell, J. D. (2003). Comparison of Ribotyping and Repetitive Extragenic Palindromic-PCR for Identification of Fecal Escherichia coli from Humans and Animals. Appl. Environ. Microbiol. 69: 1836-1839 [Abstract] [Full Text]  
  • Scott, T. M., Parveen, S., Portier, K. M., Rose, J. B., Tamplin, M. L., Farrah, S. R., Koo, A., Lukasik, J. (2003). Geographical Variation in Ribotype Profiles of Escherichia coli Isolates from Humans, Swine, Poultry, Beef, and Dairy Cattle in Florida. Appl. Environ. Microbiol. 69: 1089-1092 [Abstract] [Full Text]  
  • Jenkins, M. B., Hartel, P. G., Olexa, T. J., Stuedemann, J. A. (2003). Putative Temporal Variability of Escherichia coli Ribotypes from Yearling Steers. J. Environ. Qual. 32: 305-309 [Abstract] [Full Text]  
  • Scott, T. M., Rose, J. B., Jenkins, T. M., Farrah, S. R., Lukasik, J. (2002). Microbial Source Tracking: Current Methodology and Future Directions. Appl. Environ. Microbiol. 68: 5796-5803 [Full Text]  
  • Schaper, M., Duran, A. E., Jofre, J. (2002). Comparative Resistance of Phage Isolates of Four Genotypes of F-Specific RNA Bacteriophages to Various Inactivation Processes. Appl. Environ. Microbiol. 68: 3702-3707 [Abstract] [Full Text]  
  • Hartel, P. G., Summer, J. D., Hill, J. L., Collins, J. V., Entry, J. A., Segars, W. I. (2002). Geographic Variability of Escherichia coli Ribotypes from Animals in Idaho and Georgia. J. Environ. Qual. 31: 1273-1278 [Abstract] [Full Text]  
  • Wheeler, A. L., Hartel, P. G., Godfrey, D. G., Hill, J. L., Segars, W. I. (2002). Potential of Enterococcus faecalis as a Human Fecal Indicator for Microbial Source Tracking. J. Environ. Qual. 31: 1286-1293 [Abstract] [Full Text]  
  • Graves, A. K., Hagedorn, C., Teetor, A., Mahal, M., Booth, A. M., Reneau, R. B. Jr. (2002). Antibiotic Resistance Profiles to Determine Sources of Fecal Contamination in a Rural Virginia Watershed. J. Environ. Qual. 31: 1300-1308 [Abstract] [Full Text]  
  • Gordon, D. M., Bauer, S., Johnson, J. R. (2002). The genetic structure of Escherichia coli populations in primary and secondary habitats. Microbiology 148: 1513-1522 [Abstract] [Full Text]  
  • Gordon, D. M. (2001). Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. Microbiology 147: 1079-1085 [Full Text]  
  • Carson, C. A., Shear, B. L., Ellersieck, M. R., Asfaw, A. (2001). Identification of Fecal Escherichia coli from Humans and Animals by Ribotyping. Appl. Environ. Microbiol. 67: 1503-1507 [Abstract] [Full Text]  
  • Masseret, E, Boudeau, J, Colombel, J F, Neut, C, Desreumaux, P, Joly, B, Cortot, A, Darfeuille-Michaud, A (2001). Genetically related Escherichia coli strains associated with Crohn's disease. Gut 48: 320-325 [Abstract] [Full Text]  
  • Dombek, P. E., Johnson, L. K., Zimmerley, S. T., Sadowsky, M. J. (2000). Use of Repetitive DNA Sequences and the PCR To Differentiate Escherichia coli Isolates from Human and Animal Sources. Appl. Environ. Microbiol. 66: 2572-2577 [Abstract] [Full Text]  
  • Farnleitner, A. H., Kreuzinger, N., Kavka, G. G., Grillenberger, S., Rath, J., Mach, R. L. (2000). Simultaneous Detection and Differentiation of Escherichia coli Populations from Environmental Freshwaters by Means of Sequence Variations in a Fragment of the beta -D-Glucuronidase Gene. Appl. Environ. Microbiol. 66: 1340-1346 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»